Propionate-cultured sludge bioaugmentation to enhance methane production and micropollutant degradation in landfill leachate treatment

Khathapon Pinpatthanapong; Pornpan Panichnumsin; Chantaraporn Phalakornkule; Songkeart Phattarapattamawong; Chairat Treesubsuntorn; Nimaradee Boonapatcharoen; Kanjana Ketbuppha; Supaporn Phanwilai; Jarungwit Boonnorat

doi:10.1016/j.biortech.2022.127241

Propionate-cultured sludge bioaugmentation to enhance methane production and micropollutant degradation in landfill leachate treatment

Bioresour Technol. 2022 Jul:355:127241. doi: 10.1016/j.biortech.2022.127241. Epub 2022 Apr 27.

Authors

Khathapon Pinpatthanapong¹, Pornpan Panichnumsin², Chantaraporn Phalakornkule³, Songkeart Phattarapattamawong⁴, Chairat Treesubsuntorn⁵, Nimaradee Boonapatcharoen⁶, Kanjana Ketbuppha⁷, Supaporn Phanwilai⁸, Jarungwit Boonnorat⁹

Affiliations

¹ Department of Environmental Engineering, Faculty of Engineering, Rajamangala University of Technology Thanyaburi (RMUTT), Pathum Thani 12110, Thailand.
² Excellent Center of Waste Utilization and Management, King Mongkut's University of Technology Thonburi (KMUTT), Bangkok 10150, Thailand; National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), Pathum Thani 12120, Thailand.
³ Department of Chemical Engineering, King Mongkut's University of Technology North Bangkok (KMUTNB), Bangkok 10800, Thailand; Research Center for Circular Products and Energy, King Mongkut's University of Technology North Bangkok (KMUTNB), Bangkok 10800, Thailand.
⁴ Department of Environmental Engineering, Faculty of Engineering, King Mongkut's University of Technology Thonburi (KMUTT), Bangkok 10140, Thailand.
⁵ Division of Biotechnology, School of Bioresources and Technology, King Mongkut's University of Technology Thonburi (KMUTT), Bangkok 10150, Thailand; Remediation Laboratory, King Mongkut's University of Technology Thonburi (KMUTT), Bangkok 10150, Thailand.
⁶ Excellent Center of Waste Utilization and Management, King Mongkut's University of Technology Thonburi (KMUTT), Bangkok 10150, Thailand.
⁷ The Joint Graduate School of Energy and Environment (JGSEE), King Mongkut's University of Technology Thonburi (KMUTT), Bangkok 10140, Thailand.
⁸ Department of Knowledge of The Land for Sustainable, School of Integrated Science, Kasetsart University, Bangkok 10900, Thailand.
⁹ Department of Environmental Engineering, Faculty of Engineering, Rajamangala University of Technology Thanyaburi (RMUTT), Pathum Thani 12110, Thailand. Electronic address: jarungwit_b@rmutt.ac.th.

PMID: 35489571
DOI: 10.1016/j.biortech.2022.127241

Abstract

This research investigates the use of propionate-cultured sludge to enhance methane (CH₄) production and micropollutant biodegradation in biochemical methane potential (BMP) experiment treating landfill leachate. The experiments were carried out using non-acclimatized and acclimatized seed sludge with variable food to microorganism ratios of 1:1 and 1:2. Under the propionate-cultured sludge bioaugmentation, the concentrations of propionate-cultured sludge were varied between 10, 20, and 30 % (v/v). The acclimatized seed sludge exhibited high microbial abundance and diversity which promoted the CH₄ production and micropollutant biodegradation. The modified Gompertz model indicated that the optimal condition was the acclimatized seed sludge with 30% (v/v) propionate-cultured sludge, achieving the lag time (λ), maximum CH₄ production rate (R_max), and maximum CH₄ potential yield (P_max) of 0.57 day, 17.35 NmL/h, and 140.58 NmL/g COD. The research novelty lies in the use of propionate-cultured sludge bioaugmentation in landfill leachate treatment to enhance CH₄ production and micropollutant biodegradation.

Keywords: Acclimatized seed sludge; Anaerobic digestion; Biochemical methane potential; VFA-degrading bacteria.

MeSH terms

Anaerobiosis
Bioreactors
Methane / metabolism
Propionates
Sewage* / chemistry
Water Pollutants, Chemical* / analysis

Substances

Propionates
Sewage
Water Pollutants, Chemical
Methane