Comprehensive and Comparative Structural Glycome Analysis in Mouse Epiblast-like Cells

Federico Pecori; Hisatoshi Hanamatsu; Jun-Ichi Furukawa; Shoko Nishihara

doi:10.1007/978-1-0716-2281-0_13

Comprehensive and Comparative Structural Glycome Analysis in Mouse Epiblast-like Cells

Methods Mol Biol. 2022:2490:179-193. doi: 10.1007/978-1-0716-2281-0_13.

Authors

Federico Pecori^#¹, Hisatoshi Hanamatsu^#², Jun-Ichi Furukawa³, Shoko Nishihara^{4

5}

Affiliations

¹ Laboratory of Cell Biology, Department of Bioinformatics, Graduate School of Engineering, Soka University, Tokyo, Japan.
² Department of Advanced Clinical Glycobiology, Faculty of Medicine and Graduate School of Medicine, Hokkaido University, Sapporo, Hokkaido, Japan.
³ Department of Advanced Clinical Glycobiology, Faculty of Medicine and Graduate School of Medicine, Hokkaido University, Sapporo, Hokkaido, Japan. j_furu@med.hokudai.ac.jp.
⁴ Laboratory of Cell Biology, Department of Bioinformatics, Graduate School of Engineering, Soka University, Tokyo, Japan. shoko@soka.ac.jp.
⁵ Glycan and Life System Integration Center (GaLSIC), Soka University, Tokyo, Japan. shoko@soka.ac.jp.

^# Contributed equally.

PMID: 35486246
DOI: 10.1007/978-1-0716-2281-0_13

Abstract

Glycosylation is one of the most abundant posttranslational modifications and is involved in a wide range of cellular processes. Glycome diversity in mammals is generated by the action of over 200 distinct glycosyltransferases and related enzymes. Nevertheless, glycosylation dynamics are tightly coordinated to allow proper organismal development. Here, using mouse embryonic stem cells (mESCs) and mouse epiblast-like cells (mEpiLCs) as model systems, we describe a robust protocol that allows comprehensive and comparative structural analysis of the glycome.

Keywords: Embryonic stem cells; Epiblast-like cells; Free oligosaccharides; Glycosaminoglycans; Glycosphingolipids; Glycosylation; N-Glycans; O-Glycans; Pluripotent stem cells.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cell Line
Embryonic Stem Cells
Germ Layers*
Mammals
Mice
Mouse Embryonic Stem Cells
Pluripotent Stem Cells*