Pyrene-polyethylene glycol-modified multi-walled carbon nanotubes: Genotoxicity in V79-4 fibroblast cells

Mutat Res Genet Toxicol Environ Mutagen. 2022 Apr-May:876-877:503463. doi: 10.1016/j.mrgentox.2022.503463. Epub 2022 Feb 11.

Abstract

The genotoxicity of pyrene-polyethylene glycol-modified multi-walled carbon nanotubes (MWCNT-PyPEG), engineered as a nanoplatform for bioapplication, was evaluated. Toxicity was assessed in hamster lung fibroblast cells (V79-4). MTT and Cell Titer Blue methods were used to evaluate cell viability. Genotoxicity was measured by the comet assay and the cytokinesis-block micronucleus cytome (CBMN-Cyt) assay, and fluorescence in situ hybridization (FISH) was used to test induction of structural chromosome aberrations (clastogenic activity) and/or numerical chromosome changes (aneuploidogenic activity). Exogenous metabolic activation enzymes were used in the CBMN-Cyt and FISH tests. Only with metabolic activation, the hybrids caused chromosomal damage, by both clastogenic and aneugenic processes.

Keywords: Comet assay; Micronucleus assayMetabolic activation; Viability.

MeSH terms

  • Animals
  • Cricetinae
  • Fibroblasts
  • In Situ Hybridization, Fluorescence / methods
  • Mutagens / toxicity
  • Nanotubes, Carbon* / toxicity
  • Polyethylene Glycols / toxicity
  • Pyrenes / toxicity

Substances

  • Mutagens
  • Nanotubes, Carbon
  • Pyrenes
  • Polyethylene Glycols