Serum NMR Profiling Reveals Differential Alterations in the Lipoproteome Induced by Pfizer-BioNTech Vaccine in COVID-19 Recovered Subjects and Naïve Subjects

Veronica Ghini; Laura Maggi; Alessio Mazzoni; Michele Spinicci; Lorenzo Zammarchi; Alessandro Bartoloni; Francesco Annunziato; Paola Turano

doi:10.3389/fmolb.2022.839809

Serum NMR Profiling Reveals Differential Alterations in the Lipoproteome Induced by Pfizer-BioNTech Vaccine in COVID-19 Recovered Subjects and Naïve Subjects

Front Mol Biosci. 2022 Apr 5:9:839809. doi: 10.3389/fmolb.2022.839809. eCollection 2022.

Authors

Veronica Ghini^{1

2

3}, Laura Maggi⁴, Alessio Mazzoni⁴, Michele Spinicci^{4

5}, Lorenzo Zammarchi^{4

5}, Alessandro Bartoloni^{4

5}, Francesco Annunziato^{4

6}, Paola Turano^{1

2

3}

Affiliations

¹ Department of Chemistry, University of Florence, Florence, Italy.
² Magnetic Resonance Center (CERM), University of Florence, Florence, Italy.
³ Consorzio Interuniversitario Risonanze Magnetiche di Metallo Proteine (CIRMMP), Florence, Italy.
⁴ Department of Experimental and Clinical Medicine, University of Florence, Florence, Italy.
⁵ Infectious and Tropical Disease Unit, Careggi University Hospital, Florence, Italy.
⁶ Flow Cytometry Diagnostic Center and Immunotherapy, Careggi University Hospital, Florence, Italy.

Abstract

¹H NMR spectra of sera have been used to define the changes induced by vaccination with Pfizer-BioNTech vaccine (2 shots, 21 days apart) in 10 COVID-19-recovered subjects and 10 COVID-19-naïve subjects at different time points, starting from before vaccination, then weekly until 7 days after second injection, and finally 1 month after the second dose. The data show that vaccination does not induce any significant variation in the metabolome, whereas it causes changes at the level of lipoproteins. The effects are different in the COVID-19-recovered subjects with respect to the naïve subjects, suggesting that a previous infection reduces the vaccine modulation of the lipoproteome composition.

Keywords: NMR; SARS-CoV-2; lipoproteins; metabolomics; vaccine.