Polar metabolomics in human muscle biopsies using a liquid-liquid extraction and full-scan LC-MS

Bauke V Schomakers; Jill Hermans; Yorrick R J Jaspers; Gajja Salomons; Frédéric M Vaz; Michel van Weeghel; Riekelt H Houtkooper

doi:10.1016/j.xpro.2022.101302

Polar metabolomics in human muscle biopsies using a liquid-liquid extraction and full-scan LC-MS

STAR Protoc. 2022 Apr 16;3(2):101302. doi: 10.1016/j.xpro.2022.101302. eCollection 2022 Jun 17.

Authors

Bauke V Schomakers^{1

2}, Jill Hermans^{1

2}, Yorrick R J Jaspers¹, Gajja Salomons¹, Frédéric M Vaz^{1

2}, Michel van Weeghel^{1

2}, Riekelt H Houtkooper¹

Affiliations

¹ Laboratory Genetic Metabolic Diseases, Amsterdam UMC, Location AMC, Meibergdreef 9, AZ 1105 Amsterdam, the Netherlands.
² Core Facility Metabolomics, Amsterdam UMC, Location AMC, Meibergdreef 9, AZ 1105 Amsterdam, the Netherlands.

Abstract

We describe here a user-friendly analysis protocol for semi-targeted polar metabolomics in human muscle biopsies using Zwitterionic Hydrophilic Interaction Liquid Chromatography and high-resolution full-scan mass spectrometry. Previously, this protocol has been used for Caenorhabditis elegans. Here we show that it can be successfully applied to human muscle biopsies with minor adjustments. Summarized instructions for other matrices are also provided. As peak integration in metabolomics can be challenging, we provide expected retention times and extensive peak descriptions to aid this process. For complete details on the use and execution of this protocol, please refer to Molenaars et al. (2021).

Keywords: Health Sciences; Mass Spectrometry; Metabolism; Metabolomics.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Biopsy
Chromatography, Liquid / methods
Humans
Liquid-Liquid Extraction
Metabolomics* / methods
Muscles
Tandem Mass Spectrometry* / methods