Immunomodulatory activity of a water-soluble polysaccharide extracted from mussel on cyclophosphamide-induced immunosuppressive mice models

Xingwei Xiang; Rui Wang; Lin Chen; Yufeng Chen; Bin Zheng; Shanggui Deng; Shulai Liu; Peilong Sun; Guoxin Shen

doi:10.1038/s41538-022-00140-8

Immunomodulatory activity of a water-soluble polysaccharide extracted from mussel on cyclophosphamide-induced immunosuppressive mice models

NPJ Sci Food. 2022 Apr 27;6(1):26. doi: 10.1038/s41538-022-00140-8.

Authors

Xingwei Xiang^{1

2

3

4}, Rui Wang^{1

2

3

4}, Lin Chen⁵, Yufeng Chen^{1

2

3

4}, Bin Zheng⁶, Shanggui Deng⁶, Shulai Liu^{7

8

9

10}, Peilong Sun^{1

2

3

4}, Guoxin Shen¹¹

Affiliations

¹ College of Food Science and Technology, Zhejiang University of Technology, Hangzhou, Zhejiang, 310014, People's Republic of China.
² Key Laboratory of Marine Fishery Resources Exploitment & Utilization of Zhejiang Province, Hangzhou, 310014, China.
³ National R&D Branch Center for Pelagic Aquatic Products Processing (Hangzhou), Hangzhou, 310014, China.
⁴ Collaborative Innovation Center of Seafood Deep Processing, Dalian Polytechnic University, Dalian, 116034, China.
⁵ Sericultural and Tea Institute, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, Zhejiang, China.
⁶ Food and Pharmacy College, Zhejiang Ocean University, Zhoushan, Zhejiang, 316000, People's Republic of China.
⁷ College of Food Science and Technology, Zhejiang University of Technology, Hangzhou, Zhejiang, 310014, People's Republic of China. slliu@zjut.edu.cn.
⁸ Key Laboratory of Marine Fishery Resources Exploitment & Utilization of Zhejiang Province, Hangzhou, 310014, China. slliu@zjut.edu.cn.
⁹ National R&D Branch Center for Pelagic Aquatic Products Processing (Hangzhou), Hangzhou, 310014, China. slliu@zjut.edu.cn.
¹⁰ Collaborative Innovation Center of Seafood Deep Processing, Dalian Polytechnic University, Dalian, 116034, China. slliu@zjut.edu.cn.
¹¹ Sericultural and Tea Institute, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, Zhejiang, China. guoxinshen@gmail.com.

Abstract

This study aimed to investigate the protective effect of mussel polysaccharide (MP) on cyclophosphamide (Cy)-induced intestinal mucosal immunosuppression and microbial dysbiosis in mice. MP was shown to stimulate secretion of cytokines (SIgA, IL-2, IF-γ, IL-4, IL-10) and production of transcription factors (occludin, claudin-1, ZO-1, mucin-2, IL-2, IF-γ, IL-4, IL-10). Key proteins (p-IκB-α, p-p65) of the NF-κB pathway were upregulated after MP administration. SCFAs levels, which were decreased after the Cy treatment, were improved after treatment with MP. Furthermore, 16 S rRNA sequencing data of fecal samples revealed, through α-diversity and β-diversity analysis, that MP improved microbial community diversity and modulate the overall composition of gut microbiota. Taxonomic composition analysis showed that MP increased the abundance of probiotics species (Lactobacillus) and decreased the proportion of pathogenic species (Desulfovibrio). These findings suggested that MP has a potential immunomodulatory activity on the immunosuppressive mice.