Effective utilization of refractory dissolved organic matters in domestic sewage allows to enhanced nitrogen removal by integrated fermentation, nitrification, denitratation and anammox process

Yiling Wu; Xingxing Zhang; Junjiang Chen; Chaochao Wang; Xiaonong Zhang; Jiaqiao Chen; Tianning Cai; Wenru Liu; Xiang Li; Peng Wu

doi:10.1016/j.biortech.2022.127227

Effective utilization of refractory dissolved organic matters in domestic sewage allows to enhanced nitrogen removal by integrated fermentation, nitrification, denitratation and anammox process

Bioresour Technol. 2022 Jun:354:127227. doi: 10.1016/j.biortech.2022.127227. Epub 2022 Apr 25.

Authors

Affiliations

¹ National and Local Joint Engineering Laboratory of Municipal Sewage Resource Utilization Technology, Jiangsu Collaborative Innovation Center of Technology and Material of Water Treatment, School of Environmental Science and Engineering, Suzhou University of Science and Technology, Suzhou 215009, China.
² Environmental Microbiome and Biotechnology Lab, School of Ecological and Environmental Sciences, East China Normal University, Shanghai 200241, China.
³ National and Local Joint Engineering Laboratory of Municipal Sewage Resource Utilization Technology, Jiangsu Collaborative Innovation Center of Technology and Material of Water Treatment, School of Environmental Science and Engineering, Suzhou University of Science and Technology, Suzhou 215009, China. Electronic address: wupengniu@126.com.

PMID: 35477106
DOI: 10.1016/j.biortech.2022.127227

Abstract

To take full advantage of refractory dissolved organic matters (rDOMs) and generate sufficient nitrate for domestic sewage treatment, this study presented a novel integrated fermentation, nitrification, denitratation and anammox (IFNDA) process in a combined ABR-CSTR reactor. The results showed that an advanced total nitrogen (TN) removal efficiency of 94.1% was obtained after over 190 days operation, resulting in effluent TN concentration as low as 3.6 mg/L. The system nitrogen removal was dominated by anammox with a high proportion of 88.6%. The high conversion rate of acetic acid (54.0%) and volatile fatty acids (64.5%) from rDOMs in domestic sewage by in-situ fermentation drove efficient denitratation. Microflora analysis indicated that the enriched Commamonas (3.5%) and Longilinea (3.3%) dominated hydrolysis and acidogenesis of organics, and Methanosaeta (9.0%) obligated acetoclastic methanogenesis in two-stage fermentation process. Thauera (8.4%) and Candidatus Brocadia (2.5%) were the core bacteria for nitrogen metabolism in the IFNDA system.

Keywords: Anammox; Denitratation; Domestic sewage; Microbial community; Two-stage fermentation.

MeSH terms

Anaerobic Ammonia Oxidation
Bioreactors
Denitrification
Dissolved Organic Matter
Fermentation
Nitrification*
Nitrogen
Oxidation-Reduction
Sewage*
Wastewater

Substances

Dissolved Organic Matter
Sewage
Waste Water
Nitrogen