Characterization of biofilms formed on polystyrene microplastics (PS-MPs) on the shore of the Tuul River, Mongolia

Abstract

Microplastic (MP) surfaces are common sites for microbial colonization and promote biofilm formation in aquatic environments, resulting in changes to the surface properties of MPs and their interaction with pollutants. Although the diversity of microbial communities adhering to MPs has been well documented in aquatic environments, surface changes in MPs due to microbial colonization are still poorly understood. In this study, we aimed to evaluate the variations in the chemical structure and components of biofilms on the surface of polystyrene microplastics (PS-MPs) collected from the shore of the Tuul River in Mongolia, using micro-Fourier transform infrared (micro-FTIR) spectroscopy. We applied a spectral subtraction approach, and the differences in spectra between peroxide-treated and untreated PS-MP particles enabled us to obtain the structural features of biofilms that developed on the plastic surface. In addition, the surface photooxidation status of the sampled PS-MPs was calculated from the subtracted spectra of peroxide-treated and pristine PS-MPs. Various functional groups of N-containing organic substances from bacterial and fungal communities were detected in the obtained biofilm spectra. Based on the spectral characteristics, biofilm spectra were classified into four groups by applying principal component analysis (PCA). A wide range of carbonyl indices (CIs: 0.00-1.40) was found in the subtracted spectra between peroxide-treated and pristine PS-MPs, revealing that different levels of surface oxidation progressed by physical influences such as solar radiation and freeze-thaw cycles. Furthermore, lignocellulose and silicate were found on the PS-MP surface as allochthonous attachments. Considering the variation in residence time of PS-MPs, they attract plant residues and mineral particles through the development of biofilms and travel together in the river environment. Given that the dynamic behavior of MPs can be greatly affected by changes in their surfaces, further studies are needed to emphasize their link to organic matter dynamics.

Keywords: Biofilm-associated organic matter; Carbonyl index (CI); Infrared (IR) spectra; Principal component analysis (PCA); Urban river.