Circadian rhythms affect many aspects of a plant's metabolism including, but not limited to, photosynthesis. Here, we provide a complete protocol for determining changes in the composition of photosynthetic pigments (chlorophyll and carotenoids), and we also consider its implementation within circadian experiments. We describe how to design a circadian experiment with the goal of assessing changes in pigment composition. We then perform two consecutive approaches to track changes in pigment composition: indirect noninvasive estimation of pigment composition (by reflectance or fluorescence) followed by direct pigment analysis (by chromatography or spectrophotometry). Finally, we present several considerations regarding data analyses.
Keywords: Carotenoids; Chlorophylls; Chromatography; Internal rhythms; Noninvasive.
© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.