Dual-modal nanoplatform integrated with smartphone for hierarchical diabetic detection

Lin Huang; Yan Zhou; Yuexing Zhu; Haiyang Su; Shouzhi Yang; Lei Feng; Liang Zhao; Shanrong Liu; Kun Qian

doi:10.1016/j.bios.2022.114254

Dual-modal nanoplatform integrated with smartphone for hierarchical diabetic detection

Biosens Bioelectron. 2022 Aug 15:210:114254. doi: 10.1016/j.bios.2022.114254. Epub 2022 Apr 7.

Authors

Lin Huang¹, Yan Zhou², Yuexing Zhu³, Haiyang Su², Shouzhi Yang², Lei Feng⁴, Liang Zhao⁵, Shanrong Liu⁶, Kun Qian⁷

Affiliations

¹ Country Department of Clinical Laboratory Medicine, Shanghai Chest Hospital, Shanghai Jiao Tong University, Shanghai, 200030, PR China; Shanghai Institute of Thoracic Oncology, Shanghai Chest Hospital, Shanghai Jiao Tong University, Shanghai, 200030, PR China. Electronic address: linhuang@shsmu.edu.cn.
² School of Biomedical Engineering, Institute of Medical Robotics and Med-X Research Institute, Shanghai Jiao Tong University, Shanghai, 200030, PR China.
³ Second Military Medical University, Changhai Hospital, Department of Lab Diagnostics, Shanghai, 200433, PR China.
⁴ Instrumental Analysis Center, Shanghai Jiao Tong University, Shanghai, 201100, PR China.
⁵ School of Chemistry and Molecular Engineering, East China Normal University, Shanghai, 200241, PR China. Electronic address: lzhao@chem.ecnu.edu.cn.
⁶ Second Military Medical University, Changhai Hospital, Department of Lab Diagnostics, Shanghai, 200433, PR China. Electronic address: shanrongliu@smmu.edu.cn.
⁷ School of Biomedical Engineering, Institute of Medical Robotics and Med-X Research Institute, Shanghai Jiao Tong University, Shanghai, 200030, PR China. Electronic address: k.qian@sjtu.edu.cn.

PMID: 35462295
DOI: 10.1016/j.bios.2022.114254

Abstract

On-site screening of diabetes and precise diagnosis of diabetic complications may provide a conduit for early intervention and disease burden reduction. However, stratified metabolic analysis needs designed materials for colorimetric detection of targeted biomarkers and direct metabolic fingerprinting of the native blood. Here, an advanced dual-modal nanoplatform is constructed based on PdPtAu alloys, which serve both as the nanoenzymes in colorimetric sensing for targeted metabolite quantitation and as matrix in laser desorption/ionization mass spectrometry for untargeted metabolic fingerprinting. The platform achieved rapid glucose quantitation toward point-of-care testing of 27 participants and identified diabetic retinopathy from diabetic population with a sensitivity and specificity of 84.6%. We further assessed the generalizability of the nanoplatform for real-case applications, through the captured digital images and computing resources equipped in smartphones. The results advance the design of material-based platforms for stratified metabolic analysis and display promise to fit in the current hierarchical medical system in practice.

Keywords: Colorimetric detection; Diabetic retinopathy; Hierarchical medicine; Laser desorption/ionization mass spectrometry; Trimetallic alloys.

MeSH terms

Alloys
Biosensing Techniques*
Colorimetry
Diabetes Mellitus* / diagnosis
Diabetic Retinopathy*
Humans
Smartphone

Substances

Alloys