Formation of RNA G-wires by G4C2 repeats associated with ALS and FTD

Abstract

In the neurodegenerative disorders amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD), expansion of the G₄C₂ hexanucleotide repeat in the gene C9orf72 is a most common known cause of the disease. Here we use atomic force microscopy (AFM) and gel electrophoresis to visualize the formation of higher-order structures by RNA G₄C₂ repeats in physiologically relevant conditions. For the RNA sequence r[G₄C₂G₄], we observed G-wires with left-handed undulating features of 4.4-nm periodicity and a uniform height which is consistently higher than that of a duplex B-DNA. These higher-order structures were not degraded fully when treated with a mixture of RNase A and RNase T1. Similarly, higher-order structures were observed for sequences containing three or four G₄C₂ repeats, pointing towards their potential formation in longer sequence contexts. Our observations suggest that RNA G-quadruplex blocks and G-wires can accumulate in cells containing G₄C₂ repeat transcripts.

Keywords: ALS; C9ORF72; FTD; G(4)C(2) repeat; G-quadruplex.