Proteomic Response of Bacillus subtilis Spores under High Pressure Combined with Moderate Temperature and Random Peptide Mixture LK Treatment

Yaru Pang; Ruobin Wu; Tianlin Cui; Zequn Zhang; Li Dong; Fang Chen; Xiaosong Hu

doi:10.3390/foods11081123

Proteomic Response of Bacillus subtilis Spores under High Pressure Combined with Moderate Temperature and Random Peptide Mixture LK Treatment

Foods. 2022 Apr 13;11(8):1123. doi: 10.3390/foods11081123.

Authors

Yaru Pang^{1

2

3

4}, Ruobin Wu^{1

2

3

4}, Tianlin Cui^{1

2

3

4}, Zequn Zhang^{1

2

3

4}, Li Dong^{1

2

3

4}, Fang Chen^{1

2

3

4}, Xiaosong Hu^{1

2

3

4}

Affiliations

¹ College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China.
² China National Engineering Research Center for Fruit and Vegetable Processing, Beijing 100083, China.
³ Key Laboratory of Fruits and Vegetables Processing, Ministry of Agriculture, Beijing 100083, China.
⁴ Engineering Research Centre for Fruits and Vegetables Processing, Ministry of Education, Beijing 100083, China.

Abstract

In this study, a method of Bacillus subtilis spore inactivation under high pressure (P, 200 MPa) combined with moderate temperature (T, 80 °C) and the addition of antimicrobial peptide LK (10² μg/mL) was investigated. Spores presented cortex hydrolysis and inner membrane (IM) damage with an 8.16 log reduction in response to treatment with PT-LK, as observed by phase-contrast and inverted fluorescence microscopy and flow cytometry (FCM) analysis. Furthermore, a tandem mass tag (TMT) quantitative proteomics approach was utilized because Liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis data were used. After treatment with PT-LK, 17,017 polypeptides and 3166 proteins were detected from B. subtilis spores. Among them, 78 proteins showed significant differences in abundance between the PT-LK-treated and control groups, with 49 proteins being upregulated and 29 proteins being downregulated in the PT-LK-treated group. Genetic information processing, metabolism, cellular process, and environmental information processing were the main mechanisms of PT-LK-mediated spore inactivation.

Keywords: Bacillus subtilis spores; food sterilization; high-pressure processing; proteomic; random antimicrobial peptide mixtures; spore inactivation.

Abstract

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