Measuring Mitochondrial Calcium Fluxes in Cardiomyocytes upon Mechanical Stretch-Induced Hypertrophy

Methods Mol Biol. 2022:2475:215-222. doi: 10.1007/978-1-0716-2217-9_15.

Abstract

Calcium Ca2+ regulation is a key component of numerous cellular functions. In cardiomyocytes, Ca2+ regulates excitation-contraction coupling and influences signaling cascades involved in cell metabolism and cell survival. Prolonged dysregulation of mitochondrial Ca2+ leads to dysfunctional cardiomyocytes, apoptosis and ultimately heart failure. VEGF promotes cardiomyocyte contractility by increasing calcium transients to control the strength of the heartbeat. Here, we describe a method to measure mitochondrial Ca2+ fluxes in human ventricular cardiomocytes after inducing stretch-mediated hypertrophy in vitro.

Keywords: AC16; Calcium; Cardiomyocyte; Cyclic stretch; Hypertrophy; Mechanotransduction.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Calcium Signaling
  • Calcium* / metabolism
  • Excitation Contraction Coupling
  • Heart Ventricles
  • Humans
  • Hypertrophy / metabolism
  • Myocytes, Cardiac* / metabolism

Substances

  • Calcium