The homologous region hr4a of Autographa californica multiple nucleopolyhedrovirus specifically enhances viral early promoters

Jianan Huang; Jing Hu; Longyu Zhang; Yang Zhao; Hongying Chen; Xiaodong Xu

doi:10.1016/j.virusres.2022.198780

The homologous region hr4a of Autographa californica multiple nucleopolyhedrovirus specifically enhances viral early promoters

Virus Res. 2022 Jul 2:315:198780. doi: 10.1016/j.virusres.2022.198780. Epub 2022 Apr 16.

Authors

Jianan Huang¹, Jing Hu¹, Longyu Zhang¹, Yang Zhao¹, Hongying Chen¹, Xiaodong Xu²

Affiliations

¹ College of Life Sciences, Northwest A&F University, Yangling 712100, China.
² College of Life Sciences, Northwest A&F University, Yangling 712100, China. Electronic address: xuxd@nwsuaf.edu.cn.

PMID: 35439580
DOI: 10.1016/j.virusres.2022.198780

Abstract

Autographa californica multiple nucleocapsid polyhedrosis virus (AcMNPV) genome contains several homologous regions (hrs) which can be used as origins of viral DNA replication and transcriptional enhancers. Early studies have found that some hrs can not only stimulate viral early promoters, but also enhance gene expression driven by viral late promoters. In this study, by adding hr4a to the upstream of different types of viral promoters and enhanced green fluorescent protein (egfp) reporter gene, we investigated the enhancement effect of hr4a on eGFP expression. The results showed that hr4a increased the gene transcription controlled by early promoters, but had no enhancer effect on late promoters.

Keywords: AcMNPV; Enhancer; Promoters; hr4a.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Base Sequence
Cell Line
DNA Replication*
DNA, Viral
Nucleopolyhedroviruses
Spodoptera / genetics
Virus Replication* / genetics

Substances

DNA, Viral

Supplementary concepts

Autographa californica multiple nuclear polyhedrosis virus