How UV radiation and pH alternation impact graphene oxide mediated environmental toxicant adsorption and resulting safety characteristics - A toxicology study beyond a classic carrier effect

Abstract

Once released into water, the widely used graphene oxide (GO) is likely to adsorb classical environmental pollutants, exemplified by Microcystin-LR (MCLR) that is a representative double-bond rich liver-toxic endotoxin. While GO-mediated carrier effect is fairly predictable, the involvement of environmental factors like UV and pH may add additional level of sophistication as these factors may impact the adsorption capacity of GO to MCLR. Here, we firstly investigated the changes of GO structure under different UV-radiation durations and pH conditions with a view to establish the correlation in terms of MCLR adsorption onto GO. We demonstrated that GO reduction especially oxygen-containing groups reduction induced by UV- radiation caused the compromised adsorption MCLR capacity on GO. Besides, the higher pH decreased the non-biological MCLR adsorption to GO by reducing GO defect sites and increasing electrostatic repulsion. These abiotic discoveries were further investigated to compare the safety features of GO-MCLR complex. Under dark condition (pH = 7), we revealed the cytotoxicity of GO-MCLR to normal liver cells, which involved the ROS generation and cell ferroptosis caused by Fe²⁺ accumulation. Introduction of UV and pH alternation in environment impacted GO-mediated environmental toxicant adsorption and resulting safety characteristics, which reminded us environmental factors should not be ignored in the GO-mediated carrier effect.

Keywords: Adsorption; Combined toxicity; Graphene oxide; Microcystin-LR; Ultraviolet radiation.