Evaluation method for cell-free in situ tissue-engineered vasculature monitoring: Proof of growth and development in a canine IVC model

Goki Matsumura; Noriko Isayama; Hideki Sato

doi:10.1371/journal.pone.0267274

Evaluation method for cell-free in situ tissue-engineered vasculature monitoring: Proof of growth and development in a canine IVC model

PLoS One. 2022 Apr 18;17(4):e0267274. doi: 10.1371/journal.pone.0267274. eCollection 2022.

Authors

Goki Matsumura^{1

2}, Noriko Isayama^{2

3}, Hideki Sato⁴

Affiliations

¹ Medical Safety Management Department of Tokyo Women's Medical University, Shinjuku-ku, Tokyo, Japan.
² Department of Cardiovascular Surgery, The Heart Institute of Japan, Tokyo Women's Medical University, Shinjuku-ku, Tokyo, Japan.
³ Ueno no Mori Animal Clinic, Tokyo, Japan.
⁴ Gunze Ltd., QOL Research Center, Kyoto, Japan.

Abstract

We previously developed a non-cell-dependent biodegradable scaffold to create in situ tissue-engineered vasculature (iTEV) and tested it in a canine inferior vena cava (IVC) model. As iTEV features change dramatically during tissue generation, practical, simple, and accurate methods to evaluate iTEV are needed. The present study examined the usefulness of a novel method to evaluate iTEV growth and remodeling according to a simple formula using angiography: hepatic vein (HV) index = (IVC-HV junction angle) ÷ (π × [minimal internal iTEV diameter ÷ 2]2). HV index strongly correlated with the pressure gradient across iTEV, which tended to improve during the tissue generation period up to 12 months post-implantation. Time-course changes in HV index reflected iTEV tissue development and in-vivo characteristics, such as hemodynamic congestion. In conclusion, HV index is useful to assess iTEV graft function because it represents both the morphometrics and hemodynamics of iTEV with only diagnostic imaging data.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Dogs
Growth and Development
Hepatic Veins / diagnostic imaging
Tissue Engineering* / methods
Vena Cava, Inferior* / diagnostic imaging

Grants and funding

This work was supported in part by KAKENHI (Grant-in-aid for Scientific Research) of the Japan Society for the Promotion of Science (JSPS) (C: number 17K10739, C: number 23591878) from the Ministry of Education, Culture, Sports, Science and Technology (MEXT), Japan, and in part by an Open Research Grant from the Japan Research Promotion Society for Cardiovascular Diseases (2018 and 2019). The funders had no role in study design, data collection, analysis, decision to publish, or preparation of the manuscript.