Pediococcus acidilactici Promotes the Longevity of C. elegans by Regulating the Insulin/IGF-1 and JNK/MAPK Signaling, Fat Accumulation and Chloride Ion

Rui Hu; Yong Zhang; Weiyi Qian; Yan Leng; Yan Long; Xinjie Liu; Jinping Li; Xiangyuan Wan; Xun Wei

doi:10.3389/fnut.2022.821685

Pediococcus acidilactici Promotes the Longevity of C. elegans by Regulating the Insulin/IGF-1 and JNK/MAPK Signaling, Fat Accumulation and Chloride Ion

Front Nutr. 2022 Apr 1:9:821685. doi: 10.3389/fnut.2022.821685. eCollection 2022.

Authors

Rui Hu^{1

2}, Yong Zhang^{1

2

3}, Weiyi Qian^{1

2}, Yan Leng^{1

2

3}, Yan Long^{1

2

3}, Xinjie Liu^{1

2

3}, Jinping Li^{2

3}, Xiangyuan Wan^{1

2

3}, Xun Wei^{1

2

3}

Affiliations

¹ Zhongzhi International Institute of Agricultural Biosciences, Shunde Graduate School, Research Center of Biology and Agriculture, University of Science and Technology Beijing (USTB), Beijing, China.
² Beijing Beike Institute of Precision Medicine and Health Technology, Beijing, China.
³ Beijing Engineering Laboratory of Main Crop Bio-Tech Breeding, Beijing International Science and Technology Cooperation Base of Bio-Tech Breeding, Beijing Solidwill Sci-Tech Co., Ltd., Beijing, China.

Abstract

Probiotics are known to contribute to the anti-oxidation, immunoregulation, and aging delay. Here, we investigated the extension of lifespan by fermented pickles-origin Pediococcus acidilactici (PA) in Caenorhabditis elegans (C. elegans), and found that PA promoted a significantly extended longevity of wild-type C. elegans. The further results revealed that PA regulated the longevity via promoting the insulin/IGF-1 signaling, JNK/MAPK signaling but not TOR signaling in C. elegans, and that PA reduced the reactive oxygen species (ROS) levels and modulated expression of genes involved in fatty acids uptake and lipolysis, thus reducing the fat accumulation in C. elegans. Moreover, this study identified the nrfl-1 as the key regulator of the PA-mediated longevity, and the nrfl-1/daf-18 signaling might be activated. Further, we highlighted the roles of one chloride ion exchanger gene sulp-6 in the survival of C. elegans and other two chloride ion channel genes clh-1 and clh-4 in the prolonged lifespan by PA-feeding through the modulating expression of genes involved in inflammation. Therefore, these findings reveal the detailed and novel molecular mechanisms on the longevity of C. elegans promoted by PA.

Keywords: C. elegans; Pediococcus acidilactici; chloride ion related genes; longevity; probiotic.