A long noncoding RNA GTF2IRD2P1 suppresses cell proliferation in bladder cancer by inhibiting the Wnt/β‑catenin signaling pathway

Zhuo Huang; Hongbin Gao; Liangliang Qing; Biao Wang; Chaoyong He; Ning Luo; Chuncheng Lu; Shipeng Fan; Peng Gu; Hui Zhao

doi:10.7717/peerj.13220

A long noncoding RNA GTF2IRD2P1 suppresses cell proliferation in bladder cancer by inhibiting the Wnt/β‑catenin signaling pathway

PeerJ. 2022 Apr 11:10:e13220. doi: 10.7717/peerj.13220. eCollection 2022.

Authors

Zhuo Huang^#¹, Hongbin Gao^#^{1

2}, Liangliang Qing¹, Biao Wang¹, Chaoyong He¹, Ning Luo¹, Chuncheng Lu¹, Shipeng Fan¹, Peng Gu^{1

2}, Hui Zhao^{1

2}

Affiliations

¹ Department of Urology, First Affiliated Hospital of Kunming Medical University, Kunming Medical College, Kunming, Yunnan, People's Republic of China.
² Clinical Research Center for Chronic Kidney Disease, First Affiliated Hospital of Kunming Medical University, Kunming Medical College, Kunming, Yunnan, People's Republic of China.

^# Contributed equally.

Abstract

Background: There is growing evidence that long non-coding RNAs (LncRNAs) are key in the development of a variety of human tumors. However, the role of lncRNA GTF2IRD2P1 has not been well studied in cancer. The impact of GTF2IRD2P1 on the biological function and clinical relevance in bladder cancer is largely unknown. This study aimed to investigate the biological role of GTF2IRD2P1 in bladder evolution and carcinogenesis.

Methods: We used bioinformatics to obtain the lncRNA GTF2IRD2P1 from bladder urothelial carcinoma (BLCA) in The Cancer Genome Atlas (TCGA) database. The expression of lncRNA GTF2IRD2P1 was detected by qRT-PCR. The CCK8 assay and flow cytometry were used to detect the lncRNA GTF2IRD2P1 function on the proliferation of bladder cancer cells. A western blot was used to calculate the protein level of cell cycle proteins and Wnt signaling pathway proteins. The effect of lncRNA GTF2IRD2P1 on tumorigenesis of bladder cancer was confirmed by a xenograft nude mouse model.

Results: GTF2IRD2P1 expression was found to be lower in both human bladder cancer tissues and cell lines (UM-UC-3, RT4, and 5637), and elevated in T24 compared to the corresponding normal controls. GTF2IRD2P1 expression was also enhanced after transfection of UM-UC-3 cells with the overexpression vector. Meanwhile, overexpression of GTF2IRD2P1 inhibited the proliferation of UM-UC-3 and prolonged the cell cycle. The silencing of GTF2IRD2P1 significantly increased the proliferation and shortened the cell cycle of T24 cells and induced Wnt signaling activity to promote the progression of bladder cancer. Similarly, the transplanted tumor nude mouse model demonstrated that silencing GTF2IRD2P1 strengthens the progression of bladder cancer by targeting the Wnt signaling pathway.

Keywords: Bladder cancer; Cell cycle; Long noncoding RNA; Proliferation; Signaling pathway.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Carcinogenesis / genetics
Carcinoma, Transitional Cell*
Cell Line, Tumor
Cell Proliferation / genetics
Humans
Mice
Mice, Nude
RNA, Long Noncoding* / genetics
Urinary Bladder Neoplasms* / genetics
Wnt Signaling Pathway / genetics

Substances

RNA, Long Noncoding

Grants and funding

This study was supported by the Yunnan Provincial Department of Science and Technology-Kunming Medical University Joint Special Fund for Applied Foundations Key Project (2019FE001(-005)) and the Yunnan Provincial Department of Science and Technology-Kunming Medical University Joint Special Fund for Applied Foundations Project (2014FB031). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.