FtUGT79A15 is responsible for rutinosylation in flavonoid diglycoside biosynthesis in Fagopyrum tataricum

Huiting Xu; Zhiqiang Jiang; Zimei Lin; Qinqin Yu; Ruifeng Song; Bo Wang

doi:10.1016/j.plaphy.2022.04.004

FtUGT79A15 is responsible for rutinosylation in flavonoid diglycoside biosynthesis in Fagopyrum tataricum

Plant Physiol Biochem. 2022 Jun 15:181:33-41. doi: 10.1016/j.plaphy.2022.04.004. Epub 2022 Apr 7.

Authors

Huiting Xu¹, Zhiqiang Jiang², Zimei Lin³, Qinqin Yu⁴, Ruifeng Song⁵, Bo Wang⁶

Affiliations

¹ Guangdong Key Laboratory of Plant Molecular Breeding, College of Agriculture, South China Agricultural University, Guangzhou, 510642, China. Electronic address: huitingxu@stu.scau.edu.cn.
² Guangdong Key Laboratory of Plant Molecular Breeding, College of Agriculture, South China Agricultural University, Guangzhou, 510642, China. Electronic address: jiangzhiqiang0117@126.com.
³ Guangdong Key Laboratory of Plant Molecular Breeding, College of Agriculture, South China Agricultural University, Guangzhou, 510642, China. Electronic address: rjlsyydsa@163.com.
⁴ Guangdong Key Laboratory of Plant Molecular Breeding, College of Agriculture, South China Agricultural University, Guangzhou, 510642, China. Electronic address: qinqinyu2021@163.com.
⁵ Guangdong Key Laboratory of Plant Molecular Breeding, College of Agriculture, South China Agricultural University, Guangzhou, 510642, China. Electronic address: songruifeng@scau.edu.cn.
⁶ Guangdong Key Laboratory of Plant Molecular Breeding, College of Agriculture, South China Agricultural University, Guangzhou, 510642, China. Electronic address: bowang@scau.edu.cn.

PMID: 35428016
DOI: 10.1016/j.plaphy.2022.04.004

Abstract

Tartary buckwheat shows health benefits with its high antioxidant activity and abundant flavonoid content. However, glycosylated flavonoid accumulation patterns and their molecular basis remain unidentified in Tartary buckwheat. Here, our metabolomics analysis revealed that F3'H branching was the major flavonoid metabolic flux in Tartary buckwheat. Interestingly, metabolome results also showed that the most abundant flavonoids were mainly in the glycosylated form, including flavonoid glycosides and flavonoid diglycosides in Tartary buckwheat. However, the flavonoid glycosides glycosyltransferase (GGT) gene catalyzing the second glycosylation step of flavonoid diglycoside has not been discovered yet in Tartary buckwheat. Thus, we explored GGT genes in the transcriptome-metabolome correlation network and confirmed that FtUGT79A15 showed the rhamnosyltransferase activity to catalyze quercetin 3-O-glucoside to rutin invitro and inplanta. Overall, FtUGT79A15 was identified to involve in the flavonoid diglycoside biosynthesis pathway in Tartary buckwheat.

Keywords: Flavonoid diglycoside biosynthesis; FtUGT79A15; Rutinosylation; Tartary buckwheat.

MeSH terms

Antioxidants / metabolism
Fagopyrum* / genetics
Fagopyrum* / metabolism
Flavonoids / metabolism
Glycosylation
Rutin / metabolism

Substances

Antioxidants
Flavonoids
Rutin