Facile synthesis of peanut-like Sn-doped silica nano-adsorbent for affinity separation of proteins

Mochou Gao; Qin Liu; Yuanyuan Xue; Bao Li; Xingchi Liu; Zhenzhu Shi; Nan Liu; Xueyan Zou

doi:10.1039/d1ra08362g

Facile synthesis of peanut-like Sn-doped silica nano-adsorbent for affinity separation of proteins

RSC Adv. 2022 Feb 8;12(8):4697-4702. doi: 10.1039/d1ra08362g. eCollection 2022 Feb 3.

Authors

Mochou Gao^{1

2}, Qin Liu^{3

4

5}, Yuanyuan Xue^{3

4

5}, Bao Li^{1

3

4}, Xingchi Liu^{1

3

4}, Zhenzhu Shi^{3

4

5}, Nan Liu⁵, Xueyan Zou^{1

6

4}

Affiliations

¹ Engineering Research Center for Nanomaterials, Henan University Kaifeng 475004 China zouxueyan@henu.edu.cn 13688869875@163.com.
² National & Local Joint Engineering Research Center for Applied Technology of Hybrid Nanomaterials Kaifeng 475004 China.
³ College of Chemistry and Chemical Engineering, Henan University Kaifeng 475004 China.
⁴ State Key Laboratory of Cotton Biology Kaifeng 475004 China.
⁵ Key Laboratory for Monitor and Remediation of Heavy Metal Polluted Soils of Henan Province Jiyuan 459000 China.
⁶ Henan Ding You Agriculture Science and Technology Co., LTD. Zhengzhou 450047 China.

Abstract

A peanut-like hollow silica (denoted as p-l-hSiO₂) adsorbent is prepared in a facile method, which is composed of several silica nanospheres and has an average diameter of 22 nm, with thickness of 5 nm. Its Brunauer-Emmett-Teller (BET) surface area, pore volume and pore size are 258.9 m² g^-1, 1.56 cm³ g^-1 and 3.9 nm, respectively. Then the afforded p-l-hSiO₂/GSH adsorbent is applied to purify glutathione S-transferases-tagged (denoted as GST-tagged) proteins. It is found that the p-l-hSiO₂ adsorbent exhibits a specific adsorption, a high binding capacity (6.80 mg g^-1), good recycling performance and high recovery (90.1%) to the target proteins, showing promising potential for the affinity separation of GST-tagged proteins.