Characterization of a class II ketol-acid reductoisomerase from Mycobacterium tuberculosis

Ane Valera; Shan Wang; Reuben Carr; Laurent Trembleau; Hai Deng

doi:10.1039/d1ra08876a

Characterization of a class II ketol-acid reductoisomerase from Mycobacterium tuberculosis

RSC Adv. 2022 Apr 6;12(17):10540-10544. doi: 10.1039/d1ra08876a. eCollection 2022 Mar 31.

Authors

Ane Valera¹, Shan Wang¹, Reuben Carr², Laurent Trembleau¹, Hai Deng¹

Affiliations

¹ Department of Chemistry, University of Aberdeen Aberdeen AB24 3UE Scotland UK h.deng@abdn.ac.uk.
² Ingenza Ltd Scotland UK.

Abstract

Mycobacterium tuberculosis ketol-acid reductoisomerases have been widely studied due to their metabolic importance towards development of drug-resistant bacteria treatment. We here report the biochemical characterization of a new KARI (MtKARI-II) from a Mycobacterium tuberculosis variant with a similar kinetic profile to class I KARIs. Phylogenetic analysis suggested that MtKARI-II is clustered into a class II KARI superfamily.