Bone-like hydroxyapatite anchored on alginate microspheres for bone regeneration

Mengjie Xu; Tingting Liu; Miao Qin; Yizhu Cheng; Weiwei Lan; Xiaolian Niu; Yan Wei; Yinchun Hu; Xiaojie Lian; Liqin Zhao; Song Chen; Weiyi Chen; Di Huang

doi:10.1016/j.carbpol.2022.119330

Bone-like hydroxyapatite anchored on alginate microspheres for bone regeneration

Carbohydr Polym. 2022 Jul 1:287:119330. doi: 10.1016/j.carbpol.2022.119330. Epub 2022 Mar 8.

Authors

Mengjie Xu¹, Tingting Liu², Miao Qin³, Yizhu Cheng³, Weiwei Lan³, Xiaolian Niu³, Yan Wei⁴, Yinchun Hu¹, Xiaojie Lian¹, Liqin Zhao¹, Song Chen¹, Weiyi Chen¹, Di Huang⁵

Affiliations

¹ Department of Biomedical Engineering, Research Center for Nano-biomaterials & Regenerative Medicine, Shanxi Key Laboratory of Materials Strength & Structural Impact, College of Biomedical Engineering, Taiyuan University of Technology, Taiyuan 030024, PR China; Shanxi-Zheda Institute of Advanced Materials and Chemical Engineering, Taiyuan 030032, PR China.
² Department of Biomedical Engineering, Research Center for Nano-biomaterials & Regenerative Medicine, Shanxi Key Laboratory of Materials Strength & Structural Impact, College of Biomedical Engineering, Taiyuan University of Technology, Taiyuan 030024, PR China; Department of Laboratory Diagnosis, The 971th Hospital, Qingdao 266072, PR China.
³ Department of Biomedical Engineering, Research Center for Nano-biomaterials & Regenerative Medicine, Shanxi Key Laboratory of Materials Strength & Structural Impact, College of Biomedical Engineering, Taiyuan University of Technology, Taiyuan 030024, PR China.
⁴ Department of Biomedical Engineering, Research Center for Nano-biomaterials & Regenerative Medicine, Shanxi Key Laboratory of Materials Strength & Structural Impact, College of Biomedical Engineering, Taiyuan University of Technology, Taiyuan 030024, PR China; Shanxi-Zheda Institute of Advanced Materials and Chemical Engineering, Taiyuan 030032, PR China. Electronic address: weiyan@tyut.edu.cn.
⁵ Department of Biomedical Engineering, Research Center for Nano-biomaterials & Regenerative Medicine, Shanxi Key Laboratory of Materials Strength & Structural Impact, College of Biomedical Engineering, Taiyuan University of Technology, Taiyuan 030024, PR China; Shanxi-Zheda Institute of Advanced Materials and Chemical Engineering, Taiyuan 030032, PR China. Electronic address: huangjw2067@163.com.

PMID: 35422309
DOI: 10.1016/j.carbpol.2022.119330

Abstract

Inspired by the initial mineralization process in bone matrix vesicles (MVs), we used Dulbecco's Modified Eagle's Medium (DMEM) to establish the similar physiological environment to that in MVs for biomimetic mineralization on alginate (ALG) microspheres. The results showed that HA crystals were firstly formed and anchored on the membrane of microspheres like the initial deposition of hydroxyapatite crystals inside MVs. With the continuous growth and accumulation of mineral crystals, HA coating was finally formed on ALG microspheres. The mineralized ALG microspheres (M-ALG microspheres) show good biocompatibility and osteogenic performance. The HA coating is also conducive to the active migration of osteoblasts to the surface of M-ALG microspheres. Collectively, bone-like HA crystals anchored on ALG microspheres may provide a good prospect to promote the repair of bone defects.

Keywords: Alginate microspheres; Biomimetic mineralization; Bone matrix vesicles; Bone regeneration; Hydroxyapatite.

MeSH terms

Alginates* / chemistry
Bone Regeneration
Bone and Bones
Durapatite* / chemistry
Durapatite* / pharmacology
Microspheres

Substances

Alginates
Durapatite