The hydroxyl radicals (·OH) produced by the Fenton reaction of iron(II) and hydrogen peroxide (H2 O2 ) can oxidize the colorless 3,3',5,5'-tetramethylbenzidine (TMB) to blue oxidized TMB (Ox-TMB), resulting in a decrease in the fluorescence intensity of the reaction system and an increase in ultraviolet absorption. Ox-TMB had a visible absorption peak at 625 nm and a fluorescence peak around 420 nm. When gallic acid (GA) was added to the system, Ox-TMB was reduced to TMB, which made the color of the system disappear and the fluorescence recover. The linear ranges for determination of iron(II) were 0.5-10 μM (fluorometric) and 0.5-20 μM (colorimetric), and the detection limits were 0.25 μM (fluorometric) and 0.28 μM (colorimetric). The linear ranges for determination of GA were 0-80 μM (fluorometric) and 0-60 μM (colorimetric), and the detection limits were 0.31 μM (fluorometric) and 0.8 μM (colorimetric). The results of anti-interference experiments shew that this dual-mode assay had very good selectivity for the determination of iron(II) and GA.
Keywords: Fenton reaction; colorimetry; dual-mode determination; fluorometry; gallic acid; iron(II).
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