Effect of different anesthetic dose of pentobarbital on respiratory activity in rabbits

Xiaoyu Gu; Xuelin Zhao; Zhihao Mao; Yan Shi; Meng Xu; Maolin Cai; Fei Xie

doi:10.1016/j.compbiomed.2022.105501

Effect of different anesthetic dose of pentobarbital on respiratory activity in rabbits

Comput Biol Med. 2022 Jun:145:105501. doi: 10.1016/j.compbiomed.2022.105501. Epub 2022 Apr 7.

Authors

Xiaoyu Gu¹, Xuelin Zhao², Zhihao Mao³, Yan Shi⁴, Meng Xu⁵, Maolin Cai⁶, Fei Xie⁷

Affiliations

¹ School of Biology and Medical Engineering, Beihang University, Beijing, China.
² Department of Orthopedics, The Fourth Medical Center of PLA General Hospital, Beijing, China.
³ Department of Neurosurgery, Xintai Hospital of Traditional Chinese Medicine, Shandong, China.
⁴ School of Automation Science and Electrical Engineering, Beihang University, Beijing, China. Electronic address: shiyan@buaa.edu.cn.
⁵ Department of Orthopedics, The Fourth Medical Center of PLA General Hospital, Beijing, China. Electronic address: 13501175839@163.com.
⁶ School of Automation Science and Electrical Engineering, Beihang University, Beijing, China.
⁷ Department of Pulmonary and Critical Care Medicine, Chinese PLA General Hospital, Beijing, China. Electronic address: xiefei0522@163.com.

PMID: 35417816
DOI: 10.1016/j.compbiomed.2022.105501

Abstract

Anesthetics inhibit the respiratory muscles and even cause upper airway to collapse. Diaphragm electromyography (EMGdi) and airflow signals are usually extracted to assess the degree of respiration inhibition by anesthetics. However, the ECG interference in EMGdi affects the accuracy of its time domain and frequency domain information extraction. We studied the changes in EMGdi (left EMGdi and right EMGdi) and airflow characteristics under two pentobarbital anesthetic doses. First, we filtered out the ECG in EMGdi based on the combination of stationary wavelet transform and the positioning of ECG to obtain EMGdi without ECG interference (EMGdi_p). The effectiveness of filtering algorithm was verified by calculating the power spectrum before and after noise reduction. Second, root mean square (RMS), average rectified value (ARV), and fixed sample entropy (fSampEn) were used to quantify EMGdi (left EMGdi, left EMGdi_p and right EMGdi). Median frequency (MF) and centroid frequency (f_c) of EMGdi were calculated. Tidal volume, respiratory cycle duration and peak airflow were calculated from airflow. Finally, the average and standard deviation of these parameters for all rabbits (n = 10) were compared and analyzed under two anesthesia states. Our results indicate that anesthesia induced by an increase in pentobarbital dose leads to decrease in ventilation and EMGdi amplitude. There was no significant change in diaphragm power spectrum (MF and f_c) with the increase of anesthesia dose.

Keywords: Airflow; Pentobarbital anesthesia; Power spectrum; Quantification of diaphragm electromyography; Stationary wavelet transform.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Anesthetics* / pharmacology
Animals
Diaphragm / physiology
Electromyography / methods
Pentobarbital* / pharmacology
Rabbits
Respiratory Rate

Substances

Anesthetics
Pentobarbital