Cellobiose inhibits the release of deoxynivalenol from transformed deoxynivalenol-3-glucoside from Lactiplantibacillus plantarum

Kailin Li; Lan Wang; Dianzhen Yu; Zheng Yan; Na Liu; Aibo Wu

doi:10.1016/j.fochms.2022.100077

Cellobiose inhibits the release of deoxynivalenol from transformed deoxynivalenol-3-glucoside from Lactiplantibacillus plantarum

Food Chem (Oxf). 2022 Jan 20:4:100077. doi: 10.1016/j.fochms.2022.100077. eCollection 2022 Jul 30.

Authors

Kailin Li¹, Lan Wang¹, Dianzhen Yu¹, Zheng Yan¹, Na Liu¹, Aibo Wu¹

Affiliation

¹ SIBS-UGENT-SJTU Joint Laboratory of Mycotoxin Research, CAS Key Laboratory of Nutrition, Metabolism and Food Safety, Shanghai Institute of Nutrition and Health, University of Chinese Academy of Sciences, Chinese Academy of Sciences, China.

Abstract

The masked mycotoxin deoxynivalenol-3-glucoside (D3G) has been reported to be a detoxification product in plants, but can be hydrolyzed into its toxic precursor, deoxynivalenol (DON). Herein, we reported that Lactiplantibacillus plantarum (L. plantarum) NMM.1, isolated from Inner Mongolia raw milk, can efficiently transform D3G to DON in a short time. The global transcriptome microarray profiling of L. plantarum NMM.1 revealed differential expression of genes related to the phosphotransferase system (PTS) when D3G was used as the sole carbohydrate source. By adding an exogenous carbon source, we found that cellobiose efficiently inhibited the conversion of D3G into its precursor toxin by L. plantarum NMM.1. Overall, substrate depletion studies, transcriptome analysis, and carbohydrate intervention studies of L. plantarum NMM.1 suggested that cellobiose could be used to prevent the transformation of D3G into its free native DON by L. plantarum, thereby preventing harm to the human body.

Keywords: Cellobiose; Deoxynivalenol; Deoxynivalenol-3-glucoside; Lactiplantibacillus plantarum; Mycotoxin; Transcriptome.