Exopolysaccharides from a Scandinavian fermented milk viili increase butyric acid and Muribaculum members in the mouse gut

Takuya Yamane; Satoshi Handa; Momoko Imai; Naoki Harada; Tatsuji Sakamoto; Tetsuo Ishida; Takenori Nakagaki; Yoshihisa Nakano

doi:10.1016/j.fochms.2021.100042

Exopolysaccharides from a Scandinavian fermented milk viili increase butyric acid and Muribaculum members in the mouse gut

Food Chem (Oxf). 2021 Sep 22:3:100042. doi: 10.1016/j.fochms.2021.100042. eCollection 2021 Dec 30.

Authors

Takuya Yamane^{1

2

3}, Satoshi Handa^{1

2}, Momoko Imai^{1

4

5}, Naoki Harada², Tatsuji Sakamoto^{1

2}, Tetsuo Ishida⁶, Takenori Nakagaki³, Yoshihisa Nakano¹

Affiliations

¹ Center for Research and Development Bioresources, Organization for Research Promotion, Osaka Prefecture University, Sakai 599-8570, Japan.
² Department of Applied Life Sciences, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Sakai 599-8531, Japan.
³ Institute of Food Sciences, Nakagaki Consulting Engineer and Co Ltd Nishi-ku, Sakai 593-8328, Japan.
⁴ Faculty of Human Development, Department of Food and Nutrition Management Studies, Soai University, Osaka 559-0033, Japan.
⁵ Department of Applied Life Sciences, Graduate School of Comprehensive Rehabilitation, Osaka Prefecture University, Habikino, Osaka 583-8555, Japan.
⁶ Department of Chemistry, Biology and Marine Science, Faculty of Science, University of the Ryukyus, Nishihara, Okinawa 903-0213, Japan.

Abstract

Starter culture of viili contains lactic acid bacteria belonging to Lactococcus lactis. These bacteria secrete large polysaccharides (EPSs) into milk, resulting in a ropy texture of viili. In mouse experiments, a large dose of EPS (5-140 mg/day) has been shown to alleviate severity of artificially induced illness through modulation of the gut microbiota. The present study investigated whether supplementary amounts of EPS affects the gut microbiota of normal mouse. EPS with high glucosamine content (VEPS) was isolated from home-made viili. C57BL/6J male mice fed ordinary diet took 49 ± 1 μg VEPS/day for 28 days by drinking ad libitum tap water containing 8 μg/mL VEPS. The relative abundance of Muribaculum increased significantly by VEPS supplementation. The relative abundance of fecal butyric acid decreased in control mice, and VEPS prevented this decrease. These findings indicated that the gut microbiota can be modulated by a small dose of VEPS.

Keywords: Butylic acid; Exopolysaccharide; Intestinal microflora; Muribaculum; Viili.