Performance and mechanism of sodium percarbonate (SPC) enhancing short-chain fatty acids production from anaerobic waste activated sludge fermentation

Abstract

A novel pretreatment technique (i.e., using Sodium percarbonate, SPC) to improve the short-chain fatty acids (SCFA) production waste activated sludge (WAS) was proposed in this study. Results indicated that the maximum SCFA production of 1605.7 mg COD/L and acetic acid of 52.9% were attained at 0.2 g SPC/g TSS, being 8.4 and 2.8 times of the control (191.3 mg COD/L and 19%), respectively. Meanwhile, the optimal time for SCFA accumulation was decreased from 6d (control) to 4d (0.2 g/g TSS). Mechanism explorations unraveled that SPC largely accelerated WAS solubilization and enhanced the bioavailability of organics released from WAS. It improved enzymatic activities related to hydrolysis and acidogenesis, while suppressed the Coenzyme F₄₂₀ responsible for SCFA consumption. Illumina MiSeq sequencing analysis showed that SPC substantially enhanced the relative abundances of hydrolytic and/or acid-forming microbes. Furthermore, CO₃^- and O₂^- were the key factors to production enhancement in SPC-involved sludge fermentation.

Keywords: Anaerobic fermentation; Reactive radicals; Short-chain fatty acids (SCFA); Sodium percarbonate (SPC); Waste activated sludge (WAS).