Single molecule detection; from microscopy to sensors

Nidhi Chauhan; Kirti Saxena; Utkarsh Jain

doi:10.1016/j.ijbiomac.2022.04.038

Single molecule detection; from microscopy to sensors

Int J Biol Macromol. 2022 Jun 1;209(Pt A):1389-1401. doi: 10.1016/j.ijbiomac.2022.04.038. Epub 2022 Apr 10.

Authors

Nidhi Chauhan¹, Kirti Saxena¹, Utkarsh Jain²

Affiliations

¹ Amity Institute of Nanotechnology (AINT), Amity University Uttar Pradesh (AUUP), Noida 201313, U.P., India.
² Amity Institute of Nanotechnology (AINT), Amity University Uttar Pradesh (AUUP), Noida 201313, U.P., India. Electronic address: karshjain@gmail.com.

PMID: 35413320
DOI: 10.1016/j.ijbiomac.2022.04.038

Abstract

Single molecule detection is necessary to find out physical, chemical properties and their mechanism involved in the normal functioning of body cells. In this way, they can provide a new direction to the healthcare system. Various techniques have been developed and employed for their successful detection. Herein, we have emphasized various traditional methods as well as biosensing technology which offer single molecule sensitivity. The various methods including plasmonic resonance, nanopores, whispering gallery mode, Simoa assay and recognition tunneling are discussed in the initial part which has been followed by a discussion about biosensor-based detection. Plasmonic, SERS, CRISPR/Cas, and other types of biosensors are focused in this review and found to be highly sensitive for single molecule detection. This review provides an overview of progression in different techniques employed for single molecule detection.

Keywords: Biosensor; Detection; Microscopy; Nanopore; Plasmon; Single molecules.

Publication types

Review

MeSH terms

Biosensing Techniques* / methods
Microscopy
Nanopores*
Nanotechnology / methods