PTH2R is related to cell proliferation and migration in ovarian cancer: a multi-omics analysis of bioinformatics and experiments

Wang Xiaowei; Lu Tong; Qu Yanjun; Fan Lili

doi:10.1186/s12935-022-02566-2

PTH2R is related to cell proliferation and migration in ovarian cancer: a multi-omics analysis of bioinformatics and experiments

Cancer Cell Int. 2022 Apr 11;22(1):148. doi: 10.1186/s12935-022-02566-2.

Authors

Wang Xiaowei^#¹, Lu Tong^#², Qu Yanjun¹, Fan Lili³

Affiliations

¹ Department of Ultrasnography in Gynecology and Obstetrics, The First Affiliated Hospital of Harbin Medical University, Harbin, China.
² Department of Thoracic Surgery, The Second Affiliated Hospital of Harbin Medical University, Harbin, China.
³ Department of Children's and Adolescent Health, Public Health College, Harbin Medical University, Harbin, China. 102337@hrbmu.edu.cn.

^# Contributed equally.

Abstract

Background: Ovarian cancer is a common gynecological disease and seriously endangers women's health. Currently, there is still a lack of effective molecular markers for the diagnosis and treatment of ovarian cancer. The present study aimed to investigate the molecular markers associated with ovarian cancer.

Methods: The molecular and gene related to ovarian cancer were extracted from GEO database and TCGA database by bioinformatics, and the related genes and functions were further analyzed. The results were verified by qPCR, WB, CCK-8 and Transwell experiments.

Results: Data analysis showed that PTH2R gene was highly expressed in tumors, and 51 HUB genes were obtained. Finally, experimental verification showed that PTH2R gene was highly expressed in ovarian cancer, and PTH2R gene was involved in the proliferation, invasion and metastasis of ovarian cancer cells.

Conclusions: After experimental verification, we found that knocking down the expression of PTH2R can inhibit the proliferation, invasion and migration of tumor cells.PTH2R is expected to become a new molecular marker for ovarian cancer.

Keywords: GEO; Multi-omics; Ovarian cancer; PTH2R; TCGA.