Genome-Wide Identification and Characterisation of Cytokinin-O-Glucosyltransferase (CGT) Genes of Rice Specific to Potential Pathogens

Wadzani Palnam Dauda; Veerubommu Shanmugam; Aditya Tyagi; Amolkumar U Solanke; Vishesh Kumar; Subbaiyan Gopala Krishnan; Bishnu Maya Bashyal; Rashmi Aggarwal

doi:10.3390/plants11070917

Genome-Wide Identification and Characterisation of Cytokinin-O-Glucosyltransferase (CGT) Genes of Rice Specific to Potential Pathogens

Plants (Basel). 2022 Mar 29;11(7):917. doi: 10.3390/plants11070917.

Authors

Wadzani Palnam Dauda^{1

2}, Veerubommu Shanmugam¹, Aditya Tyagi¹, Amolkumar U Solanke³, Vishesh Kumar³, Subbaiyan Gopala Krishnan¹, Bishnu Maya Bashyal¹, Rashmi Aggarwal¹

Affiliations

¹ ICAR-Indian Agricultural Research Institute, New Delhi 110012, India.
² Crop Science Unit, Department of Agronomy, Federal University, Gashua 1005, Nigeria.
³ ICAR-National Institute for Plant Biotechnology, New Delhi 110012, India.

Abstract

Cytokinin glucosyltransferases (CGTs) are key enzymes of plants for regulating the level and function of cytokinins. In a genomic identification of rice CGTs, 41 genes with the plant secondary product glycosyltransferases (PSPG) motif of 44-amino-acid consensus sequence characteristic of plant uridine diphosphate (UDP)-glycosyltransferases (UGTs) were identified. In-silico physicochemical characterisation revealed that, though the CGTs belong to the same subfamily, they display varying molecular weights, ranging from 19.6 kDa to 59.7 kDa. The proteins were primarily acidic (87.8%) and hydrophilic (58.6%) and were observed to be distributed in the plastids (16), plasma membrane (13), mitochondria (5), and cytosol (4). Phylogenetic analysis of the CGTs revealed that their evolutionary relatedness ranged from 70-100%, and they aligned themselves into two major clusters. In a comprehensive analysis of the available transcriptomics data of rice samples representing different growth stages only the CGT, Os04g25440.1 was significantly expressed at the vegetative stage, whereas 16 other genes were highly expressed only at the reproductive growth stage. On the contrary, six genes, LOC_Os07g30610.1, LOC_Os04g25440.1, LOC_Os07g30620.1, LOC_Os04g25490.1, LOC_Os04g37820.1, and LOC_Os04g25800.1, were significantly upregulated in rice plants inoculated with Rhizoctonia solani (RS), Xoo (Xanthomonas oryzae pv. oryzae) and Mor (Magnaporthe oryzae). In a qRT-PCR analysis of rice sheath tissue susceptible to Rhizoctonia solani, Mor, and Xoo pathogens, compared to the sterile distilled water control, at 24 h post-infection only two genes displayed significant upregulation in response to all the three pathogens: LOC_Os07g30620.1 and LOC_Os04g25820.1. On the other hand, the expression of genes LOC_Os07g30610.1, LOC_Os04g25440, LOC_Os04g25490, and LOC_Os04g25800 were observed to be pathogen-specific. These genes were identified as the candidate-responsive CGT genes and could serve as potential susceptibility genes for facilitating pathogen infection.

Keywords: bacterial leaf blight (BLB); blast; cytokinin glycosyltransferases (CGTs); family 1 glycosyltransferases (GT1s); plant secondary product glycosyltransferases (PSPG); rice; sheath blight (ShB).