Carboxymethylation modification, characterization, antioxidant activity and anti-UVC ability of Sargassum fusiforme polysaccharide

Yongzhen An; Haitang Liu; Xuexiu Li; Jing Liu; Lin Chen; Xin Jin; Ting Chen; Wenqian Wang; Zhong Liu; Meiyun Zhang; Fufeng Liu

doi:10.1016/j.carres.2022.108555

Carboxymethylation modification, characterization, antioxidant activity and anti-UVC ability of Sargassum fusiforme polysaccharide

Carbohydr Res. 2022 May:515:108555. doi: 10.1016/j.carres.2022.108555. Epub 2022 Apr 2.

Authors

Yongzhen An¹, Haitang Liu², Xuexiu Li¹, Jing Liu¹, Lin Chen¹, Xin Jin¹, Ting Chen³, Wenqian Wang⁴, Zhong Liu¹, Meiyun Zhang⁵, Fufeng Liu⁶

Affiliations

¹ China Light Industry Key Laboratory of Papermaking and Biorefinery, College of Light Industry Science and Engineering, Tianjin University of Science and Technology, Tianjin, 300457, China.
² China Light Industry Key Laboratory of Papermaking and Biorefinery, College of Light Industry Science and Engineering, Tianjin University of Science and Technology, Tianjin, 300457, China; Key Laboratory of Food Nutrition and Safety, Ministry of Education, Tianjin Key Laboratory of Food Nutrition and Safety, Tianjin University of Science and Technology, Tianjin, 300457, China. Electronic address: liuhaitang@tust.edu.cn.
³ China Light Industry Key Laboratory of Papermaking and Biorefinery, College of Light Industry Science and Engineering, Tianjin University of Science and Technology, Tianjin, 300457, China; College of Bioresources Chemical and Materials Engineering, Shaanxi University of Science and Technology, Xi'an, 710021, China.
⁴ School of Biological Engineering, Tianjin University of Science & Technology, China.
⁵ College of Bioresources Chemical and Materials Engineering, Shaanxi University of Science and Technology, Xi'an, 710021, China. Electronic address: myzhang@sust.edu.cn.
⁶ School of Biological Engineering, Tianjin University of Science & Technology, China. Electronic address: fufengliu@tust.edu.cn.

PMID: 35405391
DOI: 10.1016/j.carres.2022.108555

Abstract

Taking the degree of substitution (DS) as the index, the carboxymethylation conditions of Sargassum fusiforme polysaccharide (SFP) were studied. According to the single factor experiment results, the optimum experimental conditions were obtained: sodium hydroxide concentration, 15% (20 mL); alkalization temperature, 50 °C; dosage of chloroacetic acid 1.5 g; etherification time, 2 h, and the Carboxymethyl Sargassum fusiforme polysaccharide (CSFP) with the highest DS (0.635) was obtained. And then, the physicochemical properties, structural information and bioactivity of SFP and CSFP were characterized. The SFP and CSFP were composed of four monosaccharides, with a small amount of protein, and their molecular weights to 780.2 kDa and 386.3 kDa respectively. The results of FTIR and NMR showed that the carboxymethyl was successfully grafted onto the C-4 and C-6 of sugar chain. The results of anti UVC experiment showed that SFP and CSFP had a certain negative effect on cell activity, and the degree of damage caused by UVC radiation was weakened, and the anti UVC performance of CSFP was better than that of SFP.

Keywords: Biological activity; Carboxymethylation; Characterization; Sargassum fusiforme Polysaccharide.

MeSH terms

Antioxidants / chemistry
Methylation
Molecular Weight
Polysaccharides / chemistry
Sargassum* / chemistry

Substances

Antioxidants
Polysaccharides