The Long Non-Coding RNA HOXC-AS3 Promotes Glioma Progression by Sponging miR-216 to Regulate F11R Expression

Yongshuai Li; Lu Peng; Xianwen Cao; Kun Yang; Zhen Wang; Yong Xiao; Hong Xiao; Chunfa Qian; Hongyi Liu

doi:10.3389/fonc.2022.845009

The Long Non-Coding RNA HOXC-AS3 Promotes Glioma Progression by Sponging miR-216 to Regulate F11R Expression

Front Oncol. 2022 Mar 23:12:845009. doi: 10.3389/fonc.2022.845009. eCollection 2022.

Authors

Yongshuai Li¹, Lu Peng², Xianwen Cao¹, Kun Yang¹, Zhen Wang¹, Yong Xiao¹, Hong Xiao³, Chunfa Qian¹, Hongyi Liu¹

Affiliations

¹ Department of Neurosurgery, Affiliated Nanjing Brain Hospital, Nanjing Medical University, Nanjing, China.
² Department of Clinical Laboratory, Affiliated Nanjing Brain Hospital, Nanjing Medical University, Nanjing, China.
³ Department of Neuro-Psychiatric Institute, Affiliated Nanjing Brain Hospital, Nanjing Medical University, Nanjing, China.

Abstract

HOXC cluster antisense RNA 3 (HOXC-AS3) is a long noncoding RNA (lncRNA) that plays a crucial role in various tumors; nevertheless, its role in glioma and its mechanism have not been completely elucidated. In this research, we discovered that HOXC-AS3 was over-expression in glioma cells and tissues and was associated with prognosis. Next, we determined that HOXC-AS3 targeted miR-216 as a sponge and that the F11 receptor (F11R) was the target of miR-216 by online databases analysis, qRT-PCR, and luciferase reporter assay. In addition, the rescue experiments confirmed that HOXC-AS3 regulated the expression of F11R by competitively binding miR-216 and functioning as a competing endogenous RNA (ceRNA). The intracranial glioblastoma mouse model suggested that HOXC-AS3 could promote glioma malignant progression in vivo. In summary, our study shows that the HOXC-AS3/miR-216/F11R axis plays an important role in the malignant progression of glioma, and may provide new ideas for the treatment of glioma.

Keywords: F11R; HOXC-AS3; ceRNA; glioma; miR-216.