Comparative Transcriptome Profiling Analysis Reveals the Adaptive Molecular Mechanism of Yellow-Green Leaf in Rosa beggeriana 'Aurea'

Ying Gan; Yaping Kou; Fei Yan; Xiaofei Wang; Hongqian Wang; Xiangshang Song; Min Zhang; Xin Zhao; Ruidong Jia; Hong Ge; Shuhua Yang

doi:10.3389/fpls.2022.845662

Comparative Transcriptome Profiling Analysis Reveals the Adaptive Molecular Mechanism of Yellow-Green Leaf in Rosa beggeriana 'Aurea'

Front Plant Sci. 2022 Mar 24:13:845662. doi: 10.3389/fpls.2022.845662. eCollection 2022.

Authors

Ying Gan¹, Yaping Kou¹, Fei Yan¹, Xiaofei Wang^{1

2}, Hongqian Wang¹, Xiangshang Song¹, Min Zhang¹, Xin Zhao¹, Ruidong Jia¹, Hong Ge¹, Shuhua Yang¹

Affiliations

¹ National Center of China for Flowers Improvement, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, China.
² College of Landscape Architecture and Forestry, Qingdao Agricultural University, Qingdao, China.

Abstract

Rosa beggeriana 'Aurea' is a yellow-green leaf (yl) mutant and originated from Rosa beggeriana Schrenk by ⁶⁰Co-γ irradiation, which is an important ornamental woody species. However, the molecular mechanism of the yl mutant remains unknown. Herein, comparative transcriptome profiling was performed between the yl type and normal green color type (WT) by RNA sequencing. A total of 3,372 significantly differentially expressed genes (DEGs) were identified, consisting of 1,585 upregulated genes and 1,787 downregulated genes. Genes that took part in metabolic of biological process (1,090), membrane of cellular component (728), catalytic (1,114), and binding of molecular function (840) were significantly different in transcription level. DEGs involved in chlorophyll biosynthesis, carotenoids biosynthesis, cutin, suberine, wax biosynthesis, photosynthesis, chloroplast development, photosynthesis-antenna proteins, photosystem I (PSI) and photosystem II (PSII) components, CO₂ fixation, ribosomal structure, and biogenesis related genes were downregulated. Meanwhile, linoleic acid metabolism, siroheme biosynthesis, and carbon source of pigments biosynthesis through methylerythritol 4-phosphate (MEP) pathways were upregulated. Moreover, a total of 147 putative transcription factors were signification different expression, involving NAC, WRKY, bHLH, MYB and AP2/ERF, C2H2, GRAS, and bZIP family gene. Our results showed that the disturbed pigments biosynthesis result in yl color by altering the ratio of chlorophylls and carotenoids in yl mutants. The yl mutants may evoke other metabolic pathways to compensate for the photodamage caused by the insufficient structure and function of chloroplasts, such as enhanced MEP pathways and linoleic acid metabolism against oxidative stress. This research can provide a reference for the application of leaf color mutants in the future.

Keywords: Rosa beggeriana ‘Aurea’; differentially expressed genes (DEGs); leaf color mutant; mutant mechanism; transcription profiling.