Galactooligosaccharides as a protective agent for intestinal barrier and its regulatory functions for intestinal microbiota

Geng Wang; Haidong Wang; Yuyue Jin; Zhiping Xiao; Muhammad Umar Yaqoob; Yueying Lin; Haohan Chen; Minqi Wang

doi:10.1016/j.foodres.2022.111003

Galactooligosaccharides as a protective agent for intestinal barrier and its regulatory functions for intestinal microbiota

Food Res Int. 2022 May:155:111003. doi: 10.1016/j.foodres.2022.111003. Epub 2022 Feb 22.

Authors

Geng Wang¹, Haidong Wang², Yuyue Jin³, Zhiping Xiao⁴, Muhammad Umar Yaqoob⁵, Yueying Lin⁶, Haohan Chen⁷, Minqi Wang⁸

Affiliations

¹ Key Laboratory of Molecular Animal Nutrition, Ministry of Education, College of Animal Science, Zhejiang University, Hangzhou 310058, PR China. Electronic address: wanggeng@zju.edu.cn.
² Key Laboratory of Molecular Animal Nutrition, Ministry of Education, College of Animal Science, Zhejiang University, Hangzhou 310058, PR China. Electronic address: wanghaidong@zju.edu.cn.
³ Key Laboratory of Molecular Animal Nutrition, Ministry of Education, College of Animal Science, Zhejiang University, Hangzhou 310058, PR China. Electronic address: jinyuyue@zju.edu.cn.
⁴ Key Laboratory of Molecular Animal Nutrition, Ministry of Education, College of Animal Science, Zhejiang University, Hangzhou 310058, PR China. Electronic address: xiaozp@zju.edu.cn.
⁵ Key Laboratory of Molecular Animal Nutrition, Ministry of Education, College of Animal Science, Zhejiang University, Hangzhou 310058, PR China. Electronic address: 11817048@zju.edu.cn.
⁶ Key Laboratory of Molecular Animal Nutrition, Ministry of Education, College of Animal Science, Zhejiang University, Hangzhou 310058, PR China. Electronic address: 22017011@zju.edu.cn.
⁷ Key Laboratory of Molecular Animal Nutrition, Ministry of Education, College of Animal Science, Zhejiang University, Hangzhou 310058, PR China. Electronic address: 22017099@zju.edu.cn.
⁸ Key Laboratory of Molecular Animal Nutrition, Ministry of Education, College of Animal Science, Zhejiang University, Hangzhou 310058, PR China. Electronic address: wangmq@zju.edu.cn.

PMID: 35400418
DOI: 10.1016/j.foodres.2022.111003

Abstract

Present study was conducted to comprehensively investigate the protective effects of galactooligosaccharides (GOS, 100%) against LPS-induced intestinal barrier damages, and the regulatory effect for intestinal microbes. Results showed that GOS intervention restored villi (jejunum and ileum) integrity, which were atrophic and broken in LPS-challenged mice. Electron microscopy, western blotting and immunofluorescence analysis exhibited that mice administrated with GOS showed higher expression of tight junction, which was confirmed in IPEC-J2 cells model. Meanwhile, the GOS increased the secretion of mucin and SIgA, as well as it alleviated inflammatory response caused by LPS in NF-κB dependent way. Administration of GOS could also increase the relative abundances of several specific friendly bacteria, and enhance the adaptability of intestinal microbiota. Collectively, these results indicated the potential of GOS for protecting intestine from injuries caused by stress as LPS challenge.

Keywords: Galactooligosaccharides; Inflammatory responses; Intestinal microbes; Mucin; SIgA; Tight junction.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cell Line
Gastrointestinal Microbiome*
Intestines
Lipopolysaccharides
Mice
Protective Agents

Substances

Lipopolysaccharides
Protective Agents