Evaluation of the Antidiabetic Potential of an Isolated Hydroalcoholic Fraction from the Fruit of Withania coagulans

Md Waris; Naiyer Shahzad; Saeed Saeed Al-Ghamdi; Showkat Rasool Mir; Tanuja

doi:10.4103/jpbs.JPBS_423_20

Evaluation of the Antidiabetic Potential of an Isolated Hydroalcoholic Fraction from the Fruit of Withania coagulans

J Pharm Bioallied Sci. 2021 Oct-Dec;13(4):367-372. doi: 10.4103/jpbs.JPBS_423_20. Epub 2022 Mar 4.

Authors

Md Waris^{1

2}, Naiyer Shahzad³, Saeed Saeed Al-Ghamdi³, Showkat Rasool Mir⁴, Tanuja^{1

2}

Affiliations

¹ Department of Botany, Thakur Prasad Singh (TPS) College, Patna, Magadh University, Bodh Gaya, Bihar, India.
² Department of Botany, Thakur Prasad Singh (TPS) College, Patliputra University, Patna, Bihar, India.
³ Department of Pharmacology and Toxicology, Faculty of Medicine, Umm Al-Qura University, Makkah, Saudi Arabia.
⁴ Department of Pharmacognosy and Phytochemistry, Phytopharmaceutical Research Laboratory, Faculty of Pharmacy, Jamia Hamdard, New Delhi, India.

Abstract

The hydro-alcoholic extract of Withania coagulans fruits was investigated for preliminary phytochemical screening and characterized by high-performance thin-layer chromatography. Column chromatography of the hydro-alcoholic extract of W. coagulans eluted with four different combinations of ethyl acetate and methanol yielded four fractions (WCF₀₁, WCF₀₂, WCF_03, and WCF₀₄). One of these fractions, WCF₀₂, significantly (P < 0.05) inhibited in vitro α-amylase and α-glucosidase activity with IC₅₀ values of 104.71 μg/mL and 70.79 μg/mL, respectively. WCF₀₂ further reduced blood glucose levels in comparison to control in the starch tolerance test. The extract showed a relative dose-dependent effect. It was observed that none of the extracts could delay the peak blood glucose that was achieved after 60 min of carbohydrate challenge, but these blunted the glycemic peak.

Keywords: Glycemia; Withania coagulans; high-performance thin-layer chromatography; hydro-alcoholic; α-amylase; α-glucosidase.