Sertoli cell and spermatogonial development in pigs

Yi Zheng; Qiang Gao; Tianjiao Li; Ruifang Liu; Zechao Cheng; Ming Guo; Jinhong Xiao; De Wu; Wenxian Zeng

doi:10.1186/s40104-022-00687-2

Sertoli cell and spermatogonial development in pigs

J Anim Sci Biotechnol. 2022 Apr 11;13(1):45. doi: 10.1186/s40104-022-00687-2.

Authors

Yi Zheng^#¹, Qiang Gao^#¹, Tianjiao Li¹, Ruifang Liu¹, Zechao Cheng¹, Ming Guo¹, Jinhong Xiao², De Wu³, Wenxian Zeng⁴

Affiliations

¹ Key Laboratory for Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Yangling, 712100, Shaanxi, China.
² Zhumei Porcine Breeding Corporation, Zhengzhou, Henan, China.
³ Key Laboratory for Animal Disease Resistance Nutrition of the Ministry of Education of China, Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu, 611100, Sichuan, China.
⁴ Key Laboratory for Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Yangling, 712100, Shaanxi, China. zengwenxian2015@126.com.

^# Contributed equally.

Abstract

Background: Spermatogenesis is an intricate developmental process during which undifferentiated spermatogonia, containing spermatogonial stem cells (SSCs), undergo self-renewal and differentiation to generate eventually mature spermatozoa. Spermatogenesis occurs in seminiferous tubules within the testis, and the seminiferous tubules harbor Sertoli and germ cells. Sertoli cells are an essential somatic cell type within the microenvironment that support and steer male germ cell development, whereas spermatogonia are the primitive male germ cells at the onset of spermatogenesis. While the developmental progression of Sertoli cells and spermatogonia has been well established in mice, much less is known in other mammalian species including pigs.

Results: To acquire knowledge of Sertoli cell and spermatogonial development in pigs, here we collected as many as nine ages of Duroc porcine testes from the neonate to sexual maturity, i.e., testes from 7-, 30-, 50-, 70-, 90-, 110-, 130-, 150- and 210-day-old boars, and performed histological and immunohistochemical analyses on testis sections. We first examined the development of spermatogenic cells and seminiferous tubules in porcine testes. Then, by immunofluorescence staining for marker proteins (AMH, SOX9, DBA, UCHL1, VASA, KIT, Ki67 and/or PCNA), we delved into the proliferative activity and development of Sertoli cells and of spermatogonial subtypes (pro-, undifferentiated and differentiating spermatogonia). Besides, by immunostaining for β-catenin and ZO-1, we studied the establishment of the blood-testis barrier in porcine testes.

Conclusions: In this longitudinal study, we have systematically investigated the elaborate Sertoli cell and spermatogonial developmental patterns in pigs from the neonate to sexual maturity that have so far remained largely unknown. The findings not only extend the knowledge about spermatogenesis and testicular development in pigs, but also lay the theoretical groundwork for porcine breeding and rearing.

Keywords: Pig; Sertoli cell; Spermatogenesis; Spermatogonia; Testis.

Abstract

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