Combined GWAS and QTL mapping revealed candidate genes and SNP network controlling recovery and tolerance traits associated with drought tolerance in seedling winter wheat

Ahmed Sallam; Shamseldeen Eltaher; Ahmad M Alqudah; Vikas Belamkar; P Stephen Baenziger

doi:10.1016/j.ygeno.2022.110358

Combined GWAS and QTL mapping revealed candidate genes and SNP network controlling recovery and tolerance traits associated with drought tolerance in seedling winter wheat

Genomics. 2022 May;114(3):110358. doi: 10.1016/j.ygeno.2022.110358. Epub 2022 Apr 6.

Authors

Ahmed Sallam¹, Shamseldeen Eltaher², Ahmad M Alqudah³, Vikas Belamkar⁴, P Stephen Baenziger⁴

Affiliations

¹ Department of Genetics, Faculty of Agriculture, Assiut University, Assiut, Egypt; Resources Genetics and Reproduction, Department Genebank, Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Corrensstr. 3, OT Gatersleben, D-06466 Stadt Seeland, Germany. Electronic address: amsallam@aun.edu.eg.
² Department of Plant Biotechnology, Genetic Engineering and Biotechnology Research Institute (GEBRI), University of Sadat City (USC), Egypt.
³ Department of Agroecology, Aarhus University at Flakkebjerg, Forsøgsvej 1, 4200 Slagelse, Denmark.
⁴ Department of Agronomy and Horticulture, University of Nebraska-Lincoln, Lincoln, NE 68583, USA.

PMID: 35398246
DOI: 10.1016/j.ygeno.2022.110358

Abstract

To date, very little research on drought tolerance has been conducted at the seedling stage in winter wheat. In this study, two types of traits, namely tolerance and recovery traits, associated with drought tolerance were scored in biparental mapping population (BPP) and association mapping population (A-set). The results of this study revealed no or weak significant correlation between the two types of traits. Based on GWAS and QTL mapping analyses, all QTLs associated with recovery traits were completely different from those associated with tolerance traits except one QTL in each population that was found to be associated with one tolerance trait and one recovery trait. The analysis of SNP and gene networks confirmed the results of combined GWAS and QTL mapping. One SNP marker located on the 2B chromosome (S2B_26494801) was found to be associated with recovery traits in both populations. The results of this study provided new information on understanding and improving drought tolerance in winter wheat.

Keywords: GWAS and QTL combination; Gene network; Recovery traits; Tolerance traits; Water deficit; Wheat.

MeSH terms

Drought Resistance
Droughts
Genome-Wide Association Study
Phenotype
Seedlings* / genetics
Triticum* / genetics