Effect of soil contamination with polycyclic aromatic hydrocarbons from drilling waste on germination and growth of lawn grasses

Abstract

In many studies, grasses were used to increase the biodegradation of polycyclic aromatic hydrocarbons (PAHs) in soil because they are the most common plant species on the ground level and are quite resistant to contamination with these compounds. One of the main failures in PAH remediation in soil using plant species was the negative impact on germination and seedling growth. The objective of this study was to evaluate grass seed germination and seedling growth affected by drill cuttings to determine the resistance of selected grass species to the impact of PAH and their suitability for an effective phytoremediation of soils contaminated with waste that contain compounds from this group. In the study four grass species: tall fescue (Festuca arundinacea), red fescue (Festuca rubra), perennial ryegrass (Lolium perenne) and common meadow-grass (Poa pratensis). The germination energy of all species decreased as the amount of drill cuttings increased. Among the species studied, the highest germination energy and capacity were found in Lolium perenne (54.1 and 73.2 respectively), and the lowest - in Poa pratensis (16.7 and 23.3 respectively). With an increasing amount of drill cuttings, the root and seedling height were decreased. Festuca arundinacea seedlings were distinctly the highest and had the longest roots (96.7 and 52.7, respectively), while Poa pratensis seedlings showed the significantly slowest seedling and root elongation rate (30.4 and 12.4, respectively). However, the strongest decrease in seedling height and root length compared to the control was observed in Festuca rubra. Based on IC50, the greatest tolerance to the addition of drilling waste to the substrate was found for Festuca arundinacea and Festuca rubra. The conducted investigation indicates that Festuca arundinacea and Lolium perenne are grass species that are least sensitive to drilling waste in the substrate because no significant differences were found in root length and seedling height between the control soil and the soil where a PAH dose of 5% and 10% was applied.

Keywords: Drilling waste; Germination energy; Grasses; Polycyclic aromatic hydrocarbon; Seedling growth rate; Soils.