CSFL caused by spinal dural defect is a common complication of spinal surgery, which need repair such as suture or sealants. However, low intracranial pressure symptoms, wound infection and prolonged hospital associated with pin-hole leakage or loose seal effect were often occurred after surgical suture or sealants repair. Stable, pressure resistance and high viscosity spinal dural repair patch in wet environment without suture or sealants was highly needed. Herein, a bioactive patch composed of alginate and polyacrylamide hydrogel matrix cross-linked by calcium ions, and chitosan adhesive was proposed. This fabricated patch exhibits the capabilities of promoting defect closure and good tight seal ability with the bursting pressure is more than 790 mm H2O in wet environment. In addition, the chitosan adhesive layer of the patch could inhibit the growth of bacterial in vitro, which is meaningful for the postoperative infection. Furthermore, the patch also significantly reduced the expression of GFAP, IBA-1, MBP, TNF-α, and COX-2 in early postoperative period in vivo study, exerting the effects of anti-inflammatory, analgesic and adhesion prevention. Thus, the bioactive patch expected to be applied in spinal dural repair with the good properties of withstanding high pressure, promoting defect closure and inhibiting postoperative infection.
Keywords: APS, ammonium persulfate; Alginate hydrogel; Anti-inflammatory; Bacteriostasis; CCK-8, cell counting kit-8; CSFL, cerebrospinal fluid leakage; Chitosan adhesive; Dural defect repair; EDC, 1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride; IHC, immunohistochemistry; MBAA, N,N′-Methylene bisacrylamide; NHS, N-Hydroxysuccinimide; PBS, phosphate buffered saline; SEM, scanning electron microscope; TEMED, N,N,N′,N′-Tetramethylethylenediamine.
© 2022 The Authors.