Hydroxyapatite Fabrication for Enhancing Biohydrogen Production from Glucose Dark Fermentation

Haoe Mo; Na Wang; Zhongmin Ma; Jishi Zhang; Jinlong Zhang; Lu Wang; Weifang Dong; Lihua Zang

doi:10.1021/acsomega.2c00059

Hydroxyapatite Fabrication for Enhancing Biohydrogen Production from Glucose Dark Fermentation

ACS Omega. 2022 Mar 21;7(12):10550-10558. doi: 10.1021/acsomega.2c00059. eCollection 2022 Mar 29.

Authors

Haoe Mo¹, Na Wang¹, Zhongmin Ma¹, Jishi Zhang¹, Jinlong Zhang², Lu Wang², Weifang Dong¹, Lihua Zang^{1

2}

Affiliations

¹ School of Environmental Science and Engineering, Qilu University of Technology (Shandong Academy of Science), Jinan 250353, China.
² Jilin Meihua Amino Acid Co., Ltd., Baicheng 137000, China.

Abstract

Hydroxyapatite (HA) had the effect of maintaining the pH balance of the reaction system and promoting enzyme activity. In this work, hydroxyapatite was synthesized by coprecipitation and characterized for biohydrogen (bioH₂) production from glucose. The highest bioH₂ yield obtained was 182.33 ± 2.41 mL/g glucose, amended with an optimal dosage of 400 mg/L HA, which was a 55.80% higher bioH₂ yield compared with the control group without any addition. The results indicated that HA facilitated the deterioration of organic substances and increased the concentration of soluble microbial products (SMPs). Microbial community analysis revealed that HA significantly increased the abundance of Firmicutes from 35.27% (0 mg/L, HA) to 76.41% (400 mg/L, HA), which played an essential role in bioH₂ generation. In particular, the abundance of Clostridium sensu stricto 1 increased from 15.33% (0 mg/L HA) to 45.17% (400 mg/L HA) and became the dominant bacteria. The results also indicated that HA likely improves bioH₂ production from organic wastewater in practice.