Effects of pretreatment methods on biomethane production kinetics and microbial community by solid state anaerobic digestion of sugarcane trash

Napong Ketsub; Paul Whatmore; Mahsa Abbasabadi; William O S Doherty; Prasad Kaparaju; Ian M O'Hara; Zhanying Zhang

doi:10.1016/j.biortech.2022.127112

Effects of pretreatment methods on biomethane production kinetics and microbial community by solid state anaerobic digestion of sugarcane trash

Bioresour Technol. 2022 May:352:127112. doi: 10.1016/j.biortech.2022.127112. Epub 2022 Apr 4.

Authors

Napong Ketsub¹, Paul Whatmore², Mahsa Abbasabadi³, William O S Doherty¹, Prasad Kaparaju⁴, Ian M O'Hara⁵, Zhanying Zhang⁶

Affiliations

¹ Centre for Agriculture and the Bioeconomy, Faculty of Science, Queensland University of Technology, Brisbane, Queensland 4000, Australia; School of Mechanical, Medical and Process Engineering, Faculty of Engineering, Queensland University of Technology, Brisbane, Queensland 4000, Australia.
² Bioinformatics Research Officer, Division of Research & Innovation, Queensland University of Technology, Brisbane, Queensland 4000, Australia.
³ Centre for Agriculture and the Bioeconomy, Faculty of Science, Queensland University of Technology, Brisbane, Queensland 4000, Australia; School of Biology and Environmental Sciences, Faculty of Science, Queensland University of Technology, Brisbane, Queensland 4000, Australia.
⁴ School of Engineering and Built Environment, Griffith University, Brisbane, Queensland 4111, Australia.
⁵ Centre for Agriculture and the Bioeconomy, Faculty of Science, Queensland University of Technology, Brisbane, Queensland 4000, Australia; School of Mechanical, Medical and Process Engineering, Faculty of Engineering, Queensland University of Technology, Brisbane, Queensland 4000, Australia; ARC Centre of Excellence in Synthetic Biology, QUT, Brisbane, QLD 4000, Australia.
⁶ Centre for Agriculture and the Bioeconomy, Faculty of Science, Queensland University of Technology, Brisbane, Queensland 4000, Australia; School of Mechanical, Medical and Process Engineering, Faculty of Engineering, Queensland University of Technology, Brisbane, Queensland 4000, Australia; ARC Centre of Excellence in Synthetic Biology, QUT, Brisbane, QLD 4000, Australia. Electronic address: jan.zhang@qut.edu.au.

PMID: 35381335
DOI: 10.1016/j.biortech.2022.127112

Abstract

Solid state anaerobic digestion (SS-AD) of lignocellulose is effective in improving biomethane productivity but is limited by low biomass digestibility and lack of substrate-specific working microorganisms. In this study, the effects of different pretreatment methods on biomethane production by SS-AD of sugarcane trash were studied. The biomethane production, fitted to a modified Gompertz's model, predicted a maximum methane yield of 214.2 L/kg volatile solids (VS) and productivity of 6.9 L/kg VS/day from KOH-pretreated trash, respectively. Microbial community analysis showed that bacterial community was significantly associated with volatile acids and pretreatment types while archaeal community was significantly associated with methane yield. Microbial community dynamics was revealed in SS-AD. Main genera related to pretreatment method were identified and discussed. This study generated important information on SS-AD of lignocellulosic biomass pretreated by different methods, which is useful for developing bioaugmentation strategies to improve biomethane production by SS-AD.

Keywords: Anaerobic digestion; Kinetics; Lignocellulose; Microbial community; Pretreatment.

MeSH terms

Anaerobiosis
Biofuels
Bioreactors
Edible Grain
Kinetics
Methane
Microbiota*
Saccharum*

Substances

Biofuels
Methane