Cytotoxic Activity and Antibiofilm Efficacy of Biosynthesized Silver Nanoparticles against Methicillin-Resistant Staphylococcus aureus Strains Colonizing Cell Phones

Abderrahmen Merghni; Mohamed Ali Lassoued; Emira Noumi; Ramzi Hadj Lajimi; Mohd Adnan; Maha Mastouri; Mejdi Snoussi

doi:10.1155/2022/9410024

Cytotoxic Activity and Antibiofilm Efficacy of Biosynthesized Silver Nanoparticles against Methicillin-Resistant Staphylococcus aureus Strains Colonizing Cell Phones

Can J Infect Dis Med Microbiol. 2022 Mar 25:2022:9410024. doi: 10.1155/2022/9410024. eCollection 2022.

Authors

Abderrahmen Merghni¹, Mohamed Ali Lassoued², Emira Noumi^{3

4}, Ramzi Hadj Lajimi^{5

6}, Mohd Adnan³, Maha Mastouri⁷, Mejdi Snoussi^{3

8}

Affiliations

¹ Laboratory of Antimicrobial Resistance LR99ES09, Faculty of Medicine of Tunis, University of Tunis El Manar, Tunis, Tunisia.
² Laboratory of Pharmaceutical, Chemical and Pharmacological Drug Development LR12ES09, Faculty of Pharmacy, University of Monastir, Monastir, Tunisia.
³ Department of Biology, College of Science, University of Hail, P.O. Box 2440, Ha'il, Saudi Arabia.
⁴ Laboratory of Bioresources: Integrative Biology and Recovery, High Institute of Biotechnology, University of Monastir, Monastir 5000, Tunisia.
⁵ Department of Chemistry, College of Science, University of Ha'il, P.O. Box 2440, Ha'il 81441, Saudi Arabia.
⁶ Laboratory of Water Membranes and Environmental Biotechnologies, Center of Research and Water Technologies, P. B 273, 8020 Soliman, Tunisia.
⁷ Laboratory of Transmissible Diseases and Biologically Active Substances LR99ES27, Faculty of Pharmacy, University of Monastir, Monastir, Tunisia.
⁸ Laboratory of Genetics Biodiversity and Valorisation of Bioresources, High Institute of Biotechnology, University of Monastir, Monastir 5000, Tunisia.

Abstract

The interest for green synthesis of metallic nanoparticles (NPs) has acquired particular attention due to its low toxicity and economic feasibility compared with chemical or physical process. Here we carried out an extracellular synthesis approach of silver nanoparticles (AgNPs) using dried orange peel extract. Characterization studies revealed the synthesis of 25-30 nm AgNPs with distinct morphology as observed in transmission electron microscopes. Dynamic light scattering spectroscopy and Fourier transform infrared spectroscopy analyses further characterized nanoparticles confirming their stability and the presence of functional groups. The biological properties of biosynthesized AgNPs were subsequently investigated. Our results revealed anticancer activity of biogenic silver NPs against the B16 melanoma cell line with an IC50 value of 25 µg/ml. Additionally, the developed AgNPs displayed a considerable antagonistic activity against methicillin-resistant Staphylococcus aureus (MRSA) strains colonizing cell phones, with inhibition zones between 12 and 14 mm and minimum inhibitory concentration values between 1.56 and 12.5 µg/ml. Furthermore, the AgNPs exhibited potent antibiofilm activity against MRSA strains with the percent biofilm disruption reaching 80%. Our results highlighted the efficacy of biosynthesized AgNPs against bacterial biofilms and pointed to the exploration of orange peels as a natural and cost-effective strategy.