A deep red ratiometric fluorescent probe for accurate detection of peroxynitrite in mitochondria

Rubing Han; Wei Shu; Hao Kang; Qingxia Duan; Xiaoli Zhang; Chenlu Liang; Mengxu Gao; Liren Xu; Jing Jing; Xiaoling Zhang

doi:10.1016/j.aca.2022.339652

A deep red ratiometric fluorescent probe for accurate detection of peroxynitrite in mitochondria

Anal Chim Acta. 2022 Apr 22:1203:339652. doi: 10.1016/j.aca.2022.339652. Epub 2022 Feb 25.

Authors

Rubing Han¹, Wei Shu², Hao Kang¹, Qingxia Duan¹, Xiaoli Zhang¹, Chenlu Liang¹, Mengxu Gao¹, Liren Xu¹, Jing Jing¹, Xiaoling Zhang³

Affiliations

¹ Key Laboratory of Medical Molecule Science and Pharmaceutics Engineering, Ministry of Industry and Information Technology, Key Laboratory of Cluster Science of Ministry of Education, Beijing Key Laboratory of Photo-electronic/Electro Photonic Conversion Materials, Analytical and Testing Center, School of Chemistry and Chemical Engineering, Beijing Institute of Technology, Beijing, 100081, PR China.
² School of Life Sciences and Medicine, Shandong University of Technology, Zibo 255000, PR China.
³ Key Laboratory of Medical Molecule Science and Pharmaceutics Engineering, Ministry of Industry and Information Technology, Key Laboratory of Cluster Science of Ministry of Education, Beijing Key Laboratory of Photo-electronic/Electro Photonic Conversion Materials, Analytical and Testing Center, School of Chemistry and Chemical Engineering, Beijing Institute of Technology, Beijing, 100081, PR China. Electronic address: zhangxl@bit.edu.cn.

PMID: 35361428
DOI: 10.1016/j.aca.2022.339652

Abstract

Peroxynitrite (ONOO^-) is widespread within living organisms and has been implicated in many physiological and pathological processes. Since ONOO^- is mainly produced in mitochondria, accurate detection of ONOO^- in mitochondria can help us understand its specific mechanism of action in the organism. Rather than single-wavelength emissive mitochondrial probes, ratiometric fluorescent probes with longer emission wavelength, large emission shift, and specific mitochondrial targeting properties are more likely to obtain a more accurate ONOO^- content in mitochondria. To further avoid the interference by cytoplasmic ONOO^-, we constructed a fluorescent probe MXMP with deep red emission and ratio properties, and it will be forbidden to enter the mitochondria after oxidation. In addition to its excellent selectivity and sensitivity, it shifted its fluorescence emission by up to 130 nm, with a detection limit of 84 nM.

Keywords: Deep red; Fluorescent probe; Mitochondria; Peroxynitrite; Ratio.

MeSH terms

Fluorescence
Fluorescent Dyes*
Mitochondria
Peroxynitrous Acid*

Substances

Fluorescent Dyes
Peroxynitrous Acid