Rapid-CNS2: rapid comprehensive adaptive nanopore-sequencing of CNS tumors, a proof-of-concept study

Areeba Patel; Helin Dogan; Alexander Payne; Elena Krause; Philipp Sievers; Natalie Schoebe; Daniel Schrimpf; Christina Blume; Damian Stichel; Nadine Holmes; Philipp Euskirchen; Jürgen Hench; Stephan Frank; Violaine Rosenstiel-Goidts; Miriam Ratliff; Nima Etminan; Andreas Unterberg; Christoph Dieterich; Christel Herold-Mende; Stefan M Pfister; Wolfgang Wick; Matthew Loose; Andreas von Deimling; Martin Sill; David T W Jones; Matthias Schlesner; Felix Sahm

doi:10.1007/s00401-022-02415-6

Rapid-CNS²: rapid comprehensive adaptive nanopore-sequencing of CNS tumors, a proof-of-concept study

Acta Neuropathol. 2022 May;143(5):609-612. doi: 10.1007/s00401-022-02415-6. Epub 2022 Mar 31.

Authors

Areeba Patel^#^{1

2}, Helin Dogan^#^{1

2}, Alexander Payne³, Elena Krause¹, Philipp Sievers^{1

2}, Natalie Schoebe^{1

2}, Daniel Schrimpf^{1

2}, Christina Blume^{1

2}, Damian Stichel^{1

2}, Nadine Holmes³, Philipp Euskirchen⁴, Jürgen Hench⁵, Stephan Frank⁵, Violaine Rosenstiel-Goidts⁶, Miriam Ratliff⁷, Nima Etminan⁷, Andreas Unterberg⁸, Christoph Dieterich⁹, Christel Herold-Mende⁸, Stefan M Pfister^{10

11

12}, Wolfgang Wick^{13

14}, Matthew Loose³, Andreas von Deimling^{1

2}, Martin Sill^#^{10

11}, David T W Jones^#^{10

15}, Matthias Schlesner^#¹⁶, Felix Sahm^{17

18

19}

Affiliations

¹ Department of Neuropathology, University Hospital Heidelberg, Heidelberg, Germany.
² Clinical Cooperation Unit Neuropathology, German Cancer Consortium (DKTK), German Cancer Research Center (DKFZ), Im Neuenheimer Feld 224, 69120, Heidelberg, Germany.
³ DeepSeq, School of Life Sciences, University of Nottingham, Nottingham, UK.
⁴ Department of Neurology, Charité-Universitätsmedizin Berlin, Berlin, Germany.
⁵ Division of Neuropathology, Institute of Pathology, University Hospital Basel, Basel, Switzerland.
⁶ Brain Tumor Translational Targets, German Cancer Research Center (DKFZ), Heidelberg, Germany.
⁷ Department of Neurosurgery, University Hospital Mannheim, Mannheim, Germany.
⁸ Department of Neurosurgery, University Hospital Heidelberg, Heidelberg, Germany.
⁹ Department of Cardiology, Angiology, and Pneumology, University Hospital Heidelberg, University of Heidelberg, Heidelberg, Germany.
¹⁰ Hopp Children's Cancer Center (KiTZ), Heidelberg, Germany.
¹¹ Division of Pediatric Neurooncology, German Cancer Research Center (DKFZ), German Cancer Consortium (DKTK), Heidelberg, Germany.
¹² Department of Pediatric Hematology and Oncology, Heidelberg University Hospital, Heidelberg, Germany.
¹³ Clinical Cooperation Unit Neurooncology, German Consortium for Translational Cancer Research (DKTK), German Cancer Research Center (DKFZ), Heidelberg, Germany.
¹⁴ Department of Neurology and Neurooncology Program, National Center for Tumor Diseases, Heidelberg University Hospital, Heidelberg, Germany.
¹⁵ Pediatric Glioma Research Group, German Cancer Research Center (DKFZ), Heidelberg, Germany.
¹⁶ Biomedical Informatics, Data Mining and Data Analytics, Augsburg University, Augsburg, Germany.
¹⁷ Department of Neuropathology, University Hospital Heidelberg, Heidelberg, Germany. felix.sahm@med.uni-heidelberg.de.
¹⁸ Clinical Cooperation Unit Neuropathology, German Cancer Consortium (DKTK), German Cancer Research Center (DKFZ), Im Neuenheimer Feld 224, 69120, Heidelberg, Germany. felix.sahm@med.uni-heidelberg.de.
¹⁹ Hopp Children's Cancer Center (KiTZ), Heidelberg, Germany. felix.sahm@med.uni-heidelberg.de.

^# Contributed equally.

No abstract available

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Central Nervous System Neoplasms* / genetics
High-Throughput Nucleotide Sequencing
Humans
Nanopores*
Proof of Concept Study
Sequence Analysis, DNA