Electron Transport Lipids Fold Within Membrane-Like Interfaces

Margaret M Braasch-Turi; Jordan T Koehn; Kateryna Kostenkova; Cameron Van Cleave; Jacob W Ives; Heide A Murakami; Dean C Crick; Debbie C Crans

doi:10.3389/fchem.2022.827530

Electron Transport Lipids Fold Within Membrane-Like Interfaces

Front Chem. 2022 Mar 8:10:827530. doi: 10.3389/fchem.2022.827530. eCollection 2022.

Authors

Margaret M Braasch-Turi¹, Jordan T Koehn¹, Kateryna Kostenkova¹, Cameron Van Cleave¹, Jacob W Ives¹, Heide A Murakami¹, Dean C Crick^{2

3}, Debbie C Crans^{1

2}

Affiliations

¹ Chemistry Department, Colorado State University, Fort Collins, CO, United States.
² Cell and Molecular Biology Program, Colorado State University, Fort Collins, CO, United States.
³ Microbiology, Immunology, and Pathology Department, Colorado State University, Fort Collins, CO, United States.

Abstract

Lipoquinones, such as ubiquinones (UQ) and menaquinones (MK), function as essential lipid components of the electron transport system (ETS) by shuttling electrons and protons to facilitate the production of ATP in eukaryotes and prokaryotes. Lipoquinone function in membrane systems has been widely studied, but the exact location and conformation within membranes remains controversial. Lipoquinones, such as Coenzyme Q (UQ-10), are generally depicted simply as "Q" in life science diagrams or in extended conformations in primary literature even though specific conformations are important for function in the ETS. In this study, our goal was to determine the location, orientation, and conformation of UQ-2, a truncated analog of UQ-10, in model membrane systems and to compare our results to previously studied MK-2. Herein, we first carried out a six-step synthesis to yield UQ-2 and then demonstrated that UQ-2 adopts a folded conformation in organic solvents using ¹H-¹H 2D NOESY and ROESY NMR spectroscopic studies. Similarly, using ¹H-¹H 2D NOESY NMR spectroscopic studies, UQ-2 was found to adopt a folded, U-shaped conformation within the interface of an AOT reverse micelle model membrane system. UQ-2 was located slightly closer to the surfactant-water interface compared to the more hydrophobic MK-2. In addition, Langmuir monolayer studies determined UQ-2 resided within the monolayer water-phospholipid interface causing expansion, whereas MK-2 was more likely to be compressed out and reside within the phospholipid tails. All together these results support the model that lipoquinones fold regardless of the headgroup structure but that the polarity of the headgroup influences lipoquinone location within the membrane interface. These results have implications regarding the redox activity near the interface as quinone vs. quinol forms may facilitate locomotion of lipoquinones within the membrane. The location, orientation, and conformation of lipoquinones are critical for their function in generating cellular energy within membrane ETS, and the studies described herein shed light on the behavior of lipoquinones within membrane-like environments.

Keywords: 2D NMR; Langmuir monolayer; electron transport; folded conformation; lipoquinone; membrane interface; menaquinone; ubiquinone.