Ultrastructural Analysis and Three-Dimensional Reconstruction of Plasmodesmata

Kamila Godel-Jędrychowska; Tilman Franke; Ewa Kurczyńska

doi:10.1007/978-1-0716-2132-5_4

Ultrastructural Analysis and Three-Dimensional Reconstruction of Plasmodesmata

Methods Mol Biol. 2022:2457:75-94. doi: 10.1007/978-1-0716-2132-5_4.

Authors

Kamila Godel-Jędrychowska¹, Tilman Franke², Ewa Kurczyńska³

Affiliations

¹ Faculty of Natural Sciences, Institute of Biology, Biotechnology and Environmental Protection, University of Silesia in Katowice, Katowice, Poland. kamila.godel-jedrychowska@us.edu.pl.
² Thermo Fisher Scientific, Planegg, Germany.
³ Faculty of Natural Sciences, Institute of Biology, Biotechnology and Environmental Protection, University of Silesia in Katowice, Katowice, Poland.

PMID: 35349133
DOI: 10.1007/978-1-0716-2132-5_4

Abstract

Array tomography (AT) is a new high-throughput imaging method for high-resolution imaging of ultrastructure and for 3-D reconstruction of cells and organelles. Here, we describe the entire procedure for obtaining a spatial image of the distribution of plasmodesmata (PD). As example, the protocol is applied here to reconstruct the number and arrangement of PD between cells undergoing differentiation during Arabidopsis somatic embryogenesis.

Keywords: 3-D reconstruction; Array tomography; Plasmodesmata; Ultrastructure.

MeSH terms

Image Processing, Computer-Assisted* / methods
Imaging, Three-Dimensional / methods
Plasmodesmata* / ultrastructure
Tomography / methods
Tomography, X-Ray Computed