Undesirable reflections contained in photos taken in front of glass windows or doors often degrade visual quality of the image. Separating two layers apart benefits both human and machine perception. The polarization status of the light changes after refraction or reflection, providing more observations of the scene, which can benefit the reflection separation. Different from previous works that take three or more polarization images as input, we propose to exploit physical constraints from a pair of unpolarized and polarized images to separate reflection and transmission layers in this paper. Due to the simplified capturing setup, the system is more under-determined compared to the existing polarization-based works. In order to solve this problem, we propose to estimate the semi-reflector orientation first to make the physical image formation well-posed, and then learn to reliably separate two layers using additional networks based on both physical and numerical analysis. In addition, a motion estimation network is introduced to handle the misalignment of paired input. Quantitative and qualitative experimental results show our approach performs favorably over existing polarization and single image based solutions.