NK Cell Isolation and Cytotoxicity by Radioactive Chromium Release Assay and DELFIA-EuTDA Cytotoxicity Assay

Casey W Buller; Stephen O Mathew

doi:10.1007/978-1-0716-2160-8_16

NK Cell Isolation and Cytotoxicity by Radioactive Chromium Release Assay and DELFIA-EuTDA Cytotoxicity Assay

Methods Mol Biol. 2022:2463:221-233. doi: 10.1007/978-1-0716-2160-8_16.

Authors

Casey W Buller¹, Stephen O Mathew²

Affiliations

¹ Department of Microbiology, Immunology and Genetics, University of North Texas Health Science Center, Fort Worth, TX, USA.
² Department of Microbiology, Immunology and Genetics, University of North Texas Health Science Center, Fort Worth, TX, USA. Stephen.mathew@unthsc.edu.

PMID: 35344178
DOI: 10.1007/978-1-0716-2160-8_16

Abstract

Cytotoxicity assays are important in vitro tools to measure the lysis of desired target cells via an effector immune cell of choice. Specific lysis of the target cells can be determined by labeling the target cells with a radioactive isotope or fluorescent molecule, co-incubating it with an effector cell, then measuring the release of the labeled molecule in the supernatant. Here, we describe and compare different cell cytotoxicity assays using a chromium-51 (⁵¹Cr) release and DELFIA EuTDA fluorescent assay using K562 as the target cells and peripheral blood mononuclear cell (PBMC) derived natural killer (NK) cells as the effector cells.

Keywords: Antibody dependent cellular cytotoxicity (ADCC); Chromium-51 (51Cr) release assay; DELFIA EuTDA fluorescent assay; NK cells; Natural cytotoxicity.

MeSH terms

Cytotoxicity Tests, Immunologic
Flow Cytometry
Immunologic Tests
Killer Cells, Natural*
Leukocytes, Mononuclear*