GABPB1-AS1 Promotes the Development of Osteosarcoma by Targeting SP1 and Activating the Wnt/ β-Catenin Pathway

Jingyang Chen; Meiru Bian; Lingxiao Pan; Chengdong Liu; Hanshi Yang

doi:10.1155/2022/8468896

GABPB1-AS1 Promotes the Development of Osteosarcoma by Targeting SP1 and Activating the Wnt/ β-Catenin Pathway

J Oncol. 2022 Mar 18:2022:8468896. doi: 10.1155/2022/8468896. eCollection 2022.

Authors

Jingyang Chen¹, Meiru Bian^{2

3}, Lingxiao Pan¹, Chengdong Liu⁴, Hanshi Yang^{3

5}

Affiliations

¹ Department of Orthopedics, Ningbo Medical Center Lihuili Hospital, Ningbo 315400, China.
² Department of Hematology, The Affiliated Huai'an Hospital of Xuzhou Medical University, Xuzhou, Jiangsu, China.
³ The Second People's Hospital of Huai'an, Huaian 223002, China.
⁴ Department of Orthopedics, Kunshan Geriatric Hospital, Kunshang 215324, China.
⁵ Department of Orthopedics, The Affiliated Huai'an Hospital of Xuzhou Medical University, Xuzhou, Jiangsu, China.

Abstract

In this study, the role of GABPB1-AS1 in osteosarcoma (OS) was analyzed. The expression of GABPB1-AS1 in different OS cell lines U2OS, HOS, MG63, and hFOB1.19 was detected. SiRNA GABPB1-AS1 was transfected with U2OS and HOS cell lines. The effects of GABPB1-AS1 silencing on proliferation, clonal formation, and migration of U2OS and HOS were detected by CCK-8 method, plate cloning method, and Transwell chamber. Western blot analysis was used to detect the protein levels of SP1, Wnt, β-catenin, c-Myc, and SOX2 in osteosarcoma cells. The binding relationship between GABPB1-AS1 and miR-199a-3p in OS cells was detected by a dual-luciferase reporter gene assay. Results showed that GABPB1-AS1 was higher in OS cells than that in hFOB1.19. Silencing GABPB1-AS1 inhibited the proliferation, clonal formation, migration, and epithelial-mesenchymal transformation of U2OS and HOS. There was a binding relationship between GABPB1-AS1 and miR-199a-3p in OS cells. GABPB1-AS1 mediated osteosarcoma cells via the SP1/Wnt/β-catenin signaling pathway. This study suggested that GABPB1-AS1 plays a carcinogenic role in OS through the SP1/Wnt/β-catenin signaling pathway through competitive binding and inhibition of miR-199a-3p.