Porphyrin decorated Cu2O nanocrystals for electroanalytical detection of S-Nitrosothiols

Pinghua Ling; Nuo Chen; Xinyu Sun; Xianping Gao; Linyu Wang; Pei Yang; Feng Gao

doi:10.1016/j.aca.2022.339687

Porphyrin decorated Cu₂O nanocrystals for electroanalytical detection of S-Nitrosothiols

Anal Chim Acta. 2022 Apr 15:1202:339687. doi: 10.1016/j.aca.2022.339687. Epub 2022 Mar 5.

Authors

Pinghua Ling¹, Nuo Chen², Xinyu Sun², Xianping Gao², Linyu Wang², Pei Yang², Feng Gao³

Affiliations

¹ Laboratory of Functionalized Molecular Solids, Ministry of Education, Anhui Key Laboratory of Chemo/Biosensing, College of Chemistry and Materials Science, Anhui Normal University, Wuhu, 241002, PR China. Electronic address: phling@ahnu.edu.cn.
² Laboratory of Functionalized Molecular Solids, Ministry of Education, Anhui Key Laboratory of Chemo/Biosensing, College of Chemistry and Materials Science, Anhui Normal University, Wuhu, 241002, PR China.
³ Laboratory of Functionalized Molecular Solids, Ministry of Education, Anhui Key Laboratory of Chemo/Biosensing, College of Chemistry and Materials Science, Anhui Normal University, Wuhu, 241002, PR China. Electronic address: fgao@mail.ahnu.edu.cn.

PMID: 35341533
DOI: 10.1016/j.aca.2022.339687

Abstract

S-nitrosothiols (RSNO) as the potential nitric oxide (NO) storage, transfer and delivery vehicles under physiological condition have been identified as important in a number of disease states. However, a detection and quantification of RSNO method with simple and sensitive in biological media is currently lacking. A novel electrochemical sensing platform based on ionic liquid (IL) and copper porphyrin (PorCu) decorated cuprous oxide (Cu₂O) nanocomposites was developed to detect RSNO under physiological condition. In this system, RSNO were decomposed by Cu(I) in IL/PorCu/Cu₂O to release NO, following the generated NO could be oxidized on the electrode to generate current signal. By taking advantage of the synergetic effect of IL, PorCu and Cu₂O, the sensor exhibited excellent sensitivity and linearity toward various RSNO species in phosphate buffer solution (PBS), pH 7.4. A low detection limit for N-Acetyl-3-(nitrososulfanyl)valine (SNAP), S-nitrosocysteine (CysNO) and S-nitrosoglutathione (GSNO) was 110 nM, 20 nM and 67 nM (S/N = 3), respectively. Furthermore, this method displayed good selectivity, which could be used in complex application. The detection scheme with the features of high sensitivity and ease of operation, implies the potential of IL/PorCu/Cu₂O for further various complex physiological studies.

Keywords: Cu(2)O; Electroanalytical sensing; Ionic liquid; Porphyrin; S-Nitrosothiols.

MeSH terms

Electrodes
Nanoparticles*
Nitric Oxide / chemistry
Porphyrins*
S-Nitrosothiols*

Substances

Porphyrins
S-Nitrosothiols
Nitric Oxide